Lombardi T, Montesano R, Furie M B, Silverstein S C, Orci L
Institute of Histology and Embryology, University of Geneva Medical School, Switzerland.
J Cell Sci. 1988 Oct;91 ( Pt 2):313-8. doi: 10.1242/jcs.91.2.313.
Cultured endothelial cells isolated from fenestrated capillaries express many properties characteristic of their in vivo differentiated phenotype, including the formation of a limited number of fenestrae. In this study, we have investigated whether physiological factors that control cell differentiation might regulate the surface density of fenestrae in capillary endothelial cells. We have found that treatment of the cultures with retinoic acid (10 microM) induces a more than threefold increase in the surface density of endothelial fenestrae, whereas transforming growth factor beta (TGF beta) (2 ng ml-1) causes a sevenfold decrease in the surface density of these structures. These results show that the expression of endothelial fenestrae is susceptible to bidirectional modulation by physiological signals, and suggest that retinoids and TGF beta may participate in the regulation of fenestral density of capillary endothelium in vivo.
从有孔毛细血管分离培养的内皮细胞表现出许多其体内分化表型的特征特性，包括形成数量有限的窗孔。在本研究中，我们调查了控制细胞分化的生理因素是否可能调节毛细血管内皮细胞窗孔的表面密度。我们发现，用视黄酸（10微摩尔）处理培养物可使内皮窗孔的表面密度增加三倍以上，而转化生长因子β（TGFβ）（2纳克/毫升）则使这些结构的表面密度降低七倍。这些结果表明，内皮窗孔的表达易受生理信号的双向调节，并提示类视黄醇和TGFβ可能参与体内毛细血管内皮窗孔密度的调节。
