Warsaw University of Life Sciences - SGGW, Warsaw, Poland.
Institute of Biochemistry and Biophysics, Polish Academy of Sciences, Warsaw, Poland.
Biochem Soc Trans. 2020 Aug 28;48(4):1337-1352. doi: 10.1042/BST20190712.
The protein kinase-like clan/superfamily is a large group of regulatory, signaling and biosynthetic enzymes that were historically regarded as typically eukaryotic proteins, although bacterial members have also been known for a long time. In this review, we explore the diversity of bacterial protein kinase like families, and discuss functional versatility of these enzymes, both the ones acting within the bacterial cell, and those acting within eukaryotic cells as effectors during infection. We focus on novel bacterial kinase-like families discovered in the last five years. A bioinformatics perspective is held here, hence sequence and structure comparison overview is presented, and also a comparison of genomic neighbourhoods of the families. We perform a phylum-level census of the families. Also, we discuss apparent pseudokinases that turned out to perform alternative catalytic functions by repurposing their atypical kinase-like active sites. We also highlight some 'unpopular' kinase-like families that await characterisation.
蛋白激酶样家族/超家族是一大类调节、信号和生物合成酶，历史上被认为是典型的真核蛋白，尽管很久以前就已经知道细菌中也有成员。在这篇综述中，我们探讨了细菌蛋白激酶样家族的多样性，并讨论了这些酶的功能多样性，包括在细菌细胞内发挥作用的酶，以及在感染过程中作为效应物在真核细胞内发挥作用的酶。我们专注于过去五年中发现的新型细菌激酶样家族。这里采用了生物信息学的视角，因此呈现了序列和结构比较概述，以及家族基因组邻近性的比较。我们对家族进行了门水平的普查。此外，我们还讨论了明显的伪激酶，它们通过重新利用其非典型激酶样活性位点来执行替代催化功能。我们还强调了一些等待表征的“不受欢迎”的激酶样家族。
