Siberian Federal University, 79 Svobodny pr., 660041 Krasnoyarsk, Russian Federation; Institute of Biophysics SB RAS, Federal Research Center "Krasnoyarsk Science Center SB RAS", 50/50 Akademgorodok, Krasnoyarsk 660036, Russian Federation.
Siberian Federal University, 79 Svobodny pr., 660041 Krasnoyarsk, Russian Federation; Institute of Biophysics SB RAS, Federal Research Center "Krasnoyarsk Science Center SB RAS", 50/50 Akademgorodok, Krasnoyarsk 660036, Russian Federation.
Int J Biol Macromol. 2020 Dec 1;164:121-130. doi: 10.1016/j.ijbiomac.2020.07.095. Epub 2020 Jul 14.
The study addresses the growth of the wild-type strain Cupriavidus necator B-10646 and synthesis of polyhydroxyalkanoates by this strain on media containing plant oils with different compositions of fatty acids: palm, Siberian oilseed, and refined and unrefined sunflower seed oils. The study showed that the best carbon substrate was palm oil. Comparison of fatty acid compositions of the starting oils and unutilized residual substrates showed that C. necator B-10646 cells consumed the fatty acids from palm oil evenly while in experiments with other oils, they utilized polyenoic fatty acids first. Higher production parameters of the culture were obtained by preparation of emulsified oil medium using Tween 80 and sodium cocoyl glutamate as emulsifiers. All polyhydroxyalkanoate specimens were terpolymers that contained 3-hydroxybutyrate as the major component and minor amounts of 3-hydroxyvalerate (0.9-1.9 mol%) and 3-hydroxyhexanoate (0.5-1.1 mol%). Molecular weight of polyhydroxyalkanoate specimens depended on the type of plant oil and emulsifier.
该研究探讨了野生型菌株 Cupriavidus necator B-10646 的生长以及该菌株在含有不同脂肪酸组成的植物油培养基上合成聚羟基烷酸酯的情况：棕榈油、西伯利亚油籽和精制及未精制葵花籽油。研究表明，棕榈油是最佳的碳源。起始油和未利用的残余基质的脂肪酸组成比较表明，C. necator B-10646 细胞均匀地消耗棕榈油中的脂肪酸，而在其他油的实验中，它们首先利用多烯酸。使用 Tween 80 和椰油酰谷氨酸钠作为乳化剂制备乳化油培养基，可以获得更高的培养生产参数。所有聚羟基烷酸酯标本均为共聚物，含有 3-羟基丁酸酯作为主要成分和少量 3-羟基戊酸酯（0.9-1.9 mol%）和 3-羟基己酸酯（0.5-1.1 mol%）。聚羟基烷酸酯标本的分子量取决于植物油和乳化剂的类型。
