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N Biotechnol. 2013 Jan 25;30(2):192-5. doi: 10.1016/j.nbt.2012.05.002. Epub 2012 May 23.
In the global context of increased concerns for our environment, the use of bioplastics as a replacement for existing petroleum-based polymers is an important challenge. Indeed, bioplastics hardly meet economical and technical constraints. One, of the most promising among currently studied bioplastics, is the polyhydroxyalkanoate (PHA). To circumvent the economical issue for this particular biopolymer one solution can be the enhancement of the overall productivity by the improvement of the nutritional medium of the microorganism producing the biopolymer. Thus, several nutrition media, supplemented or not with sodium glutamate, were tested for the growth and the PHA production by Cupriavidus necator DSM 545 strain. The most efficient for the biomass and the PHA production improvement were found to be the Luria broth (LB) and the Bonnarme's media, both supplemented with 10 g/L sodium glutamate. Hence the overall productivity was 33 times enhanced comparing to traditional cultivation methods. These results open a new route for the PHA production by C. necator which appears to be more suitable on a rich, or enriched, medium with no limiting factors.
在全球对环境日益关注的背景下，使用生物塑料替代现有石油基聚合物是一项重要的挑战。事实上，生物塑料几乎不能满足经济和技术方面的限制。目前研究中最有前途的生物塑料之一是聚羟基烷酸酯（PHA）。为了解决这种特殊生物聚合物的经济性问题，可以通过改善生产生物聚合物的微生物的营养培养基来提高其整体生产力。因此，测试了几种营养培养基，这些培养基或不添加谷氨酸钠，以促进铜绿假单胞菌 DSM 545 菌株的生长和 PHA 生产。发现最有利于提高生物量和 PHA 生产的是补充了 10 g/L 谷氨酸钠的 LB 培养基和 Bonnarme 培养基。因此，与传统培养方法相比，整体生产力提高了 33 倍。这些结果为铜绿假单胞菌的 PHA 生产开辟了新途径，表明其在丰富或富化的培养基中更适合，没有限制因素。
