Mi Guohua, Shi Huanxian, Yang Min, Wang Cunjin, Hao Hong, Fan Jun
School of Chemical Engineering, Northwest University, Xi'an 710069, PR China.
School of Chemical Engineering, Northwest University, Xi'an 710069, PR China.
Spectrochim Acta A Mol Biomol Spectrosc. 2020 Nov 5;241:118673. doi: 10.1016/j.saa.2020.118673. Epub 2020 Jul 2.
Doxorubicin hydrochloride (DOX) is an effective anthracycline anticancer drug. However, the exceeded taken up could induce several side-effects such as cardiotoxicity, alopecia. Therefore, the level of DOX needs to be closely monitored to avoid the occurrence of its side-effects. Herein, we report a novel core CuInSe - shell ZnS quantum dots (CuInSe@ZnS, QDs) and Ag nanoparticles (NPs) fluorescence sensor based on the surface plasmon resonance effect (SPR) of Ag NPs. The CuInSe@ZnS QDs were prepared by water phase reflux method with the 3-mercaptopropionic acid (MPA) as stabilizer and ligand. The fluorescence intensity of CuInSe@ZnS QDs/Ag NPs significantly reduced by DOX, which is mainly based on the electrostatic interaction between the DOX and fluorescence sensors. The inhibition of photoluminescence (ln F/F) was linearly relationship to the concentration of DOX in the range of 2-100 μM with the detection limit as low as 0.05 μM. The as-prepared sensor has a high selectivity and sensitivity to DOX. Furthermore, the new sensor has been successfully applied to the determination of DOX in human serum samples with satisfactory results. Our work provides a clue for developing a novel CuInSe@ZnS QDs/Ag NPs based fluorescence sensor for DOX detection.
盐酸阿霉素（DOX）是一种有效的蒽环类抗癌药物。然而，过量摄入会引发多种副作用，如心脏毒性、脱发等。因此，需要密切监测DOX的水平以避免其副作用的发生。在此，我们报道了一种基于银纳米颗粒（Ag NPs）表面等离子体共振效应（SPR）的新型核-壳结构铜铟硒量子点（CuInSe@ZnS，QDs）与Ag NPs荧光传感器。以3-巯基丙酸（MPA）作为稳定剂和配体，采用水相回流法制备了CuInSe@ZnS量子点。DOX显著降低了CuInSe@ZnS量子点/Ag NPs的荧光强度，这主要基于DOX与荧光传感器之间的静电相互作用。在2-100 μM范围内，光致发光抑制率（ln F/F）与DOX浓度呈线性关系，检测限低至0.05 μM。所制备的传感器对DOX具有高选择性和高灵敏度。此外，该新型传感器已成功应用于人体血清样品中DOX的测定，结果令人满意。我们的工作为开发一种基于CuInSe@ZnS量子点/Ag NPs的新型DOX检测荧光传感器提供了思路。
