使用抗生物素蛋白-生物素复合物通过荧光显微镜对肌动蛋白和肌球蛋白进行定位。
Use of the avidin-biotin complex for the localization of actin and myosin with fluorescence microscopy.
作者信息
Heggeness M H, Ash J F
出版信息
J Cell Biol. 1977 Jun;73(3):783-8. doi: 10.1083/jcb.73.3.783.
Abstract
A new indirect method for fluorescence localization of proteins making use of the avidin-biotin complex is described. We have prepared both a biotin-modified rabbit heavy meromyosin (BHMM) and a biotin-modified antibody to a smooth muscle myosin. After fixation, cells can be treated with either BHMM, which binds to actin, or the biotinyl antibody, which binds to myosin. In a second step the cell are treated with a fluorescent derivative of avidin (Fl-avidin) which binds to the biotinyl proteins and thus indirectly reveals the location of the cellular action or myosin.
摘要
本文描述了一种利用抗生物素蛋白-生物素复合物进行蛋白质荧光定位的新间接方法。我们制备了生物素修饰的兔重酶解肌球蛋白（BHMM）和生物素修饰的平滑肌肌球蛋白抗体。固定后，细胞可用与肌动蛋白结合的BHMM或与肌球蛋白结合的生物素化抗体处理。第二步，用抗生物素蛋白的荧光衍生物（荧光抗生物素蛋白）处理细胞，该衍生物与生物素化蛋白结合，从而间接显示细胞肌动蛋白或肌球蛋白的位置。
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