Yamamoto K, Tokunaga M, Sutoh K, Wakabayashi T, Sekine T
J Mol Biol. 1985 May 25;183(2):287-90. doi: 10.1016/0022-2836(85)90222-0.
The location of the single cysteinyl residue of the alkali light chain on the myosin head was determined by electron microscopy. The cysteinyl residue of isolated alkali light chain 2 was biotinylated and the light chain was exchanged with that of heavy meromyosin in 4.7 M-NH4Cl. Avidin was attached to the biotin in the heavy meromyosin and the complex was rotary shadowed and observed in the electron microscope. The distance from the head-rod junction to the centre of avidin was 8(+/- 3) nm (mean value +/- standard deviation: n = 105).
