Respiratory Cellular and Molecular Biology, Woolcock Institute of Medical Research, The University of Sydney, Sydney, NSW 2037, Australia.
Advanced Tissue Engineering and Stem Cell Biology Group, School of Life Sciences, Faculty of Science, University of Technology Sydney, Sydney, NSW 2007, Australia.
Int J Mol Sci. 2020 Jul 15;21(14):4990. doi: 10.3390/ijms21144990.
Cancer-derived extracellular vesicles are known to play a role in the progression of the disease. In this rapidly-growing field, there are many reports of phenotypic changes in cells following exposure to cancer-derived extracellular vesicles. This study examines the protein contents of vesicles derived from three well-known breast cancer cell lines, MCF-7, MDA-MB-231 and T47D, using peptide-centric LC-MS/MS and cytokine multiplex immunoassay analysis to understand the molecular basis of these changes. Through these techniques a large number of proteins within these vesicles were identified. A large proportion of these proteins are known to be important in cancer formation and progression and associated with cancer signaling, angiogenesis, metastasis and invasion and immune regulation. This highlights the importance of extracellular vesicles (EVs) in cancer communications and shows some of the mechanisms the vesicles use to assist in cancer progression.
癌症来源的细胞外囊泡被认为在疾病的进展中起作用。在这个快速发展的领域中，有许多报告表明，细胞在暴露于癌症来源的细胞外囊泡后会发生表型变化。本研究使用肽质组学 LC-MS/MS 和细胞因子多重免疫分析检测了三种知名乳腺癌细胞系 MCF-7、MDA-MB-231 和 T47D 来源的囊泡中的蛋白含量，以了解这些变化的分子基础。通过这些技术，鉴定出了这些囊泡中的大量蛋白。其中很大一部分蛋白已知在癌症的形成和进展中非常重要，与癌症信号转导、血管生成、转移和侵袭以及免疫调节有关。这凸显了细胞外囊泡 (EVs) 在癌症通讯中的重要性，并展示了囊泡用于协助癌症进展的一些机制。
