MSPRL, FAS Division of Science, Harvard University, Cambridge, MA, 02138, USA.
Department of Biology, Northeastern University, Boston, MA, 02115, USA.
Genome Biol. 2018 Oct 22;19(1):161. doi: 10.1186/s13059-018-1547-5.
Some exciting biological questions require quantifying thousands of proteins in single cells. To achieve this goal, we develop Single Cell ProtEomics by Mass Spectrometry (SCoPE-MS) and validate its ability to identify distinct human cancer cell types based on their proteomes. We use SCoPE-MS to quantify over a thousand proteins in differentiating mouse embryonic stem cells. The single-cell proteomes enable us to deconstruct cell populations and infer protein abundance relationships. Comparison between single-cell proteomes and transcriptomes indicates coordinated mRNA and protein covariation, yet many genes exhibit functionally concerted and distinct regulatory patterns at the mRNA and the protein level.
有些令人兴奋的生物学问题需要在单细胞中定量数千种蛋白质。为了实现这一目标，我们开发了基于质谱的单细胞蛋白质组学（SCoPE-MS），并验证了其根据蛋白质组鉴定不同人类癌细胞类型的能力。我们使用 SCoPE-MS 对分化中的小鼠胚胎干细胞中的超过一千种蛋白质进行定量。单细胞蛋白质组使我们能够对细胞群体进行解构，并推断蛋白质丰度关系。单细胞蛋白质组和转录组之间的比较表明 mRNA 和蛋白质协同变化，但许多基因在 mRNA 和蛋白质水平上表现出功能协调和不同的调控模式。
