人细胞色素b5还原酶（DIA1）的克隆及染色体定位

Cloning and chromosomal mapping of human cytochrome b5 reductase (DIA1).

作者信息

Bull P C, Shephard E A, Povey S, Santisteban I, Phillips I R

机构信息

Department of Biochemistry, University College London.

出版信息

Ann Hum Genet. 1988 Oct;52(4):263-8. doi: 10.1111/j.1469-1809.1988.tb01105.x.

DOI:10.1111/j.1469-1809.1988.tb01105.x

PMID:3268037

Abstract

We have isolated a cDNA clone that codes for human cytochrome b5 reductase. The cDNA was used to analyse, by Southern-blot hybridization, DNA isolated from a panel of 11 independent human-rodent somatic cell hybrids. The results indicate that cytochrome b5 reductase is encoded by a single gene located on human chromosome 22.

摘要

我们分离出了一个编码人细胞色素b5还原酶的cDNA克隆。利用该cDNA，通过Southern印迹杂交分析了从11个独立的人-啮齿动物体细胞杂种组中分离出的DNA。结果表明，细胞色素b5还原酶由位于人类22号染色体上的单个基因编码。