Junien C, Vibert M, Weil D, Van-Cong N, Kaplan J C
Hum Genet. 1978 Jun 27;42(3):233-9. doi: 10.1007/BF00291301.
NADH-cytochrome b5 reductase (DIA1, EC. 1.6.2.2) from human fibroblasts and from Chinese hamster cells, both identified by immunologic studies, were clearly distinguished after polyacrylamide gel isoelectro-focusing followed by staining for NADH diaphorase activity. In thirteen independent man-hamster hybrids, the human enzyme DIA1 presented a positive correlation with the human chromosome G22. Eight hybrids were DIA1(+) G22(+) and five hybrids were DIA1(-) G22(-). These data agree with the recent assignment of DIA1 to chromosome G22 by Fisher et al. (1977a). We assume that this newly assigned locus codes for both soluble and microsomal forms of NADH-cytochrome b5 reductase.
通过免疫研究鉴定的来自人成纤维细胞和中国仓鼠细胞的NADH-细胞色素b5还原酶（DIA1，EC. 1.6.2.2），在聚丙烯酰胺凝胶等电聚焦后，经NADH黄递酶活性染色，可明显区分开来。在13个独立的人-仓鼠杂种中，人酶DIA1与人类染色体G22呈正相关。8个杂种为DIA1(+) G22(+)，5个杂种为DIA1(-) G22(-)。这些数据与Fisher等人（1977a）最近将DIA1定位于染色体G22的结果一致。我们假设这个新定位的基因座编码NADH-细胞色素b5还原酶的可溶性和微粒体形式。
