Servicio de Microbiología. Hospital General Universitario de Alicante. Instituto de Investigación Sanitaria y Biomédica de Alicante-ISABIAL, Alicante, España.
Servicio de Microbiología. Hospital General Universitario de Alicante. Instituto de Investigación Sanitaria y Biomédica de Alicante-ISABIAL, Alicante, España.
Enferm Infecc Microbiol Clin (Engl Ed). 2021 Apr;39(4):184-187. doi: 10.1016/j.eimc.2020.05.023. Epub 2020 Jul 15.
Clostridioides difficile infection (CDI) has become a global healthcare challenge due to increases in its incidence and mortality rates. Faecal microbiota transfer (FMT) is postulated as a protocol to prevent CDI recurrence.
A donor faecal sample and patient faecal samples (pre-FMT and post-FMT) were analysed. The r16S gene was amplified and sequenced by NGS, and its diversity and taxonomy composition were examined.
Microbial richness increased in post-FMT samples, and the β diversity studies grouped the samples into two clusters. One included the non-pathological samples (donor and pre-FMT samples), and the other included the pathological sample. The results obtained by Qiime2 and Bioconductor were similar.
The analysis showed an increase in taxonomic diversity after the FMT, which suggests its usefulness. Moreover, these results showed that standardisation of bioinformatics analysis is key.
由于艰难梭菌感染（CDI）的发病率和死亡率不断上升，它已成为全球医疗保健的挑战。粪便微生物群转移（FMT）被认为是预防 CDI 复发的方案。
分析供体粪便样本和患者粪便样本（FMT 前和 FMT 后）。通过 NGS 扩增和测序 r16S 基因，并检查其多样性和分类组成。
FMT 后样本中的微生物丰富度增加，β多样性研究将样本分为两个聚类。一个聚类包括非病理性样本（供体和 FMT 前样本），另一个聚类包括病理性样本。Qiime2 和 Bioconductor 得到的结果相似。
分析表明 FMT 后分类多样性增加，提示其有用性。此外，这些结果表明生物信息学分析的标准化是关键。
