University of Applied Sciences and Arts Northwestern Switzerland, School of Life Sciences, Hofackerstrasse 30, CH-4132, Muttenz, Switzerland; Swiss Federal Institute of Technology (ETH Zürich), Institute of Biogeochemistry and Pollution Dynamics, Department of Environmental Systems Science, CH-8092, Zürich, Switzerland.
University of Applied Sciences and Arts Northwestern Switzerland, School of Life Sciences, Hofackerstrasse 30, CH-4132, Muttenz, Switzerland.
Chemosphere. 2020 Dec;260:127542. doi: 10.1016/j.chemosphere.2020.127542. Epub 2020 Jul 6.
Bees are exposed to endocrine active insecticides. Here we assessed expressional alteration of marker genes indicative of endocrine effects in the brain of honey bees. We exposed foragers to chlorpyrifos, cypermethrin and thiacloprid and assessed the expression of genes after exposure for 24 h, 48 h and 72 h. Chlorpyrifos caused the strongest expressional changes at 24 h characterized by induction of vitellogenin, major royal jelly protein (mrjp) 2 and 3, insulin-like peptide (ilp1), alpha-glucosidase (hbg3) and sima, and down-regulation of buffy. Cypermethrin caused minor induction of mrjp1, mrjp2, mmp1 and ilp1. The sima transcript showed down-regulation at 48 h and up-regulation at 72 h. Exposure to thiacloprid caused down-regulation of vitellogenin, mrjp1 and sima at 24 h, and hbg3 at 72 h, as well as induction of ilp1 at 48 h. The buffy transcript was down-regulated at 24 h and up-regulated at 48 h. Despite compound-specific expression patterns, each insecticide altered the expression of some of the suggested endocrine system related genes. Our study suggests that expressional changes of genes prominently expressed in nurse or forager bees, including down-regulation of buffy and mrjps and up-regulation of hbg3 and ilp1 may serve as indicators for endocrine activity of insecticides in foragers.
蜜蜂会接触到具有内分泌活性的杀虫剂。在这里，我们评估了指示蜜蜂大脑内分泌效应的标记基因的表达变化。我们使采集蜂接触氯菊酯、氯氰菊酯和噻虫啉，并在暴露 24、48 和 72 小时后评估基因的表达。氯菊酯在 24 小时时引起最强的表达变化，表现为卵黄蛋白原、主要蜂王浆蛋白 (mrjp)2 和 3、胰岛素样肽 (ilp1)、α-葡萄糖苷酶 (hbg3) 和 sima 的诱导，以及 buffy 的下调。氯氰菊酯引起 mrjp1、mrjp2、mmp1 和 ilp1 的轻微诱导。sima 转录本在 48 小时时下调，72 小时时上调。噻虫啉暴露在 24 小时时导致卵黄蛋白原、mrjp1 和 sima 下调，72 小时时 hbg3 下调，48 小时时 ilp1 诱导。buffy 转录本在 24 小时时下调，48 小时时上调。尽管存在化合物特异性表达模式，但每种杀虫剂都改变了一些建议的与内分泌系统相关基因的表达。我们的研究表明，在工蜂或采集蜂中高度表达的基因的表达变化，包括 buffy 和 mrjps 的下调以及 hbg3 和 ilp1 的上调，可能作为采集蜂中杀虫剂内分泌活性的指标。
