University of Applied Sciences and Arts Northwestern Switzerland, School of Life Sciences, Hofackerstrasse 30, CH-4132, Muttenz, Switzerland; Swiss Federal Institute of Technology Zürich (ETH Zürich), Department of Environmental Systems Science, Institute of Biogeochemistry and Pollution Dynamics, CH-8092, Zürich, Switzerland.
Genexa AG, Dienerstrasse 7, CH-8004, Zürich, Switzerland.
Environ Pollut. 2020 Apr;259:113715. doi: 10.1016/j.envpol.2019.113715. Epub 2019 Dec 4.
Cypermethrin is a frequently used insecticide in agriculture and households but its chronic and molecular effects are poorly known are . Here we describe effects of sublethal cypermethrin exposure on the global transcriptome in the brain of honey bees determined by RNA-sequencing. Exposure for 48 h to 0.3 ng/bee cypermethrin (3 ng/mL sucrose solution) causes 38 differentially expressed genes (DEGs), of which 29 are up-regulated and 9 down-regulated. Exposure to 3 ng/bee causes differential expression of 265 DEGs (209 up-, 56 down-regulated). Among the 24 DEGs shared by both concentrations are genes encoding muscular structure, muscular processes and esterase B1. Functional analysis (GO term analysis) confirms the enrichment of muscular development, structure and function among the 89 and 35 significantly altered GO terms at the low and high concentration, respectively. Up-regulation of nine DEGs determined by RT-qPCR showed a good correlation with RNA-sequence data. Among them are genes including esterase B1, titin, twitchin, mucin-19, insulin like growth factor binding protein, golgin like protein and helix loop protein. Our study demonstrates for the first time molecular effects of cypermethrin at environmental concentrations, which include expressional induction of genes encoding muscular and cellular processes and metabolism enzymes. Further studies should demonstrate the physiological consequences in bees.
氯菊酯是农业和家庭中常用的杀虫剂，但它的慢性和分子效应知之甚少。在这里，我们通过 RNA 测序描述了亚致死浓度的氯菊酯暴露对蜜蜂大脑中全局转录组的影响。暴露于 0.3ng/bee（3ng/mL 蔗糖溶液）氯菊酯 48 小时会导致 38 个差异表达基因（DEGs），其中 29 个上调，9 个下调。暴露于 3ng/bee 会导致 265 个 DEGs 的差异表达（209 个上调，56 个下调）。在这两个浓度共有的 24 个 DEGs 中，有编码肌肉结构、肌肉过程和酯酶 B1 的基因。功能分析（GO 术语分析）证实，在低浓度和高浓度下，分别有 89 个和 35 个显著改变的 GO 术语富集了肌肉发育、结构和功能。通过 RT-qPCR 确定的九个 DEGs 的上调与 RNA-seq 数据具有良好的相关性。其中包括酯酶 B1、titin、twitchin、粘蛋白 19、胰岛素样生长因子结合蛋白、高尔基体蛋白和螺旋环蛋白等基因。我们的研究首次证明了环境浓度下氯菊酯的分子效应，包括编码肌肉和细胞过程以及代谢酶的基因表达诱导。进一步的研究应该证明蜜蜂的生理后果。
