Department of Biomedical Engineering, National Cerebral and Cardiovascular Center (NCVC) Research Institute, 6-1 Kishibe-Shimmachi, Suita, Osaka, Japan.
Department of Plastic and Reconstructive Surgery, Graduate School of Medicine, Kyoto University, 54 Shogoin, Kawahara-cho, Sakyo-ku, Kyoto, Japan.
Biomed Mater Eng. 2020;31(4):203-210. doi: 10.3233/BME-201103.
Adipose tissue engineering has been studied as an alternative to current options for breast reconstruction, such as lipofilling, flap reconstruction, and silicone implants. Previously, we demonstrated that a poly(L-lactic acid) mesh containing a collagen sponge, containing neither cells nor growth factors, could be filled with the regenerated adipose tissues when implanted in rodent models. However, the main factor contributing to adipogenesis remained unclear.
We aimed to clarify whether adipogenesis can be achieved by the space provided by the mesh or by the bioactivity of collagen.
A three-dimensional (3D) poly(lactic acid) (PLA) frame, which was stiff enough to maintain its shape, was fabricated by 3D printing. The frame with (PLA+ColI) or without (PLA only) a type I collagen hydrogel was implanted in the inguinal region of rats for up to 12 months. Adipose tissue regeneration in the PLA only and PLA+ColI groups was evaluated histologically.
The 3D PLA frame maintained its structure for 12 months in vivo and oil red O (ORO)-positive adipose tissues were regenerated in the frame. No significant difference in the ORO-positive area was detected between the PLA only and PLA+ColI groups.
The space supported by the frame was a key factor in adipogenesis in vivo.
脂肪组织工程已被研究作为当前乳房重建选择的替代方案，例如脂肪填充、皮瓣重建和硅胶植入物。以前，我们证明了一种含有胶原海绵的聚（L-丙交酯）网，既不含有细胞也不含有生长因子，可以在啮齿动物模型中植入时填充再生的脂肪组织。然而，促成脂肪生成的主要因素仍不清楚。
我们旨在阐明脂肪生成是否可以通过网格提供的空间或胶原的生物活性来实现。
通过 3D 打印制造了足够坚硬以保持其形状的三维（3D）聚乳酸（PLA）框架。将带有（PLA+ColI）或不带有（仅 PLA）I 型胶原水凝胶的框架植入大鼠腹股沟区域长达 12 个月。通过组织学评估仅 PLA 和 PLA+ColI 组中的脂肪组织再生。
3D PLA 框架在体内保持其结构长达 12 个月，并且在框架中再生了油红 O（ORO）阳性的脂肪组织。在仅 PLA 和 PLA+ColI 组之间，ORO 阳性区域没有明显差异。
框架提供的空间是体内脂肪生成的关键因素。
