Department of Plastic and Reconstructive Surgery, Graduate School of Medicine, Kyoto University, Sakyou-ku, Kyoto, Japan.
Department of Plastic and Reconstructive Surgery, National Hospital Organization Kyoto Medical Center, Fushimiku, Kyoto, Japan.
J Tissue Eng Regen Med. 2020 Jul;14(7):920-930. doi: 10.1002/term.3041. Epub 2020 Jun 3.
Adipose tissue regeneration in breast cancer patients without additional growth factors or adipose-tissue-derived stromal cells is desirable because of the possibility of recurrence and metastasis. We report that a poly-L-lactic acid (PLLA) mesh implant containing a collagen sponge (CS) maintained the internal space in vivo for up to 12 months and substituted for adipose tissue. We developed a PLLA capsule that maintained the internal space longer than that of PLLA mesh and compared adipose tissue formation at 12 and 24 months after implantation between the PLLA mesh with CS implant and the PLLA capsule implant with or without CS in a rabbit model. After 12 months, all implants maintained the internal space, and the adipose tissue that formed in all implant groups was larger than that in the control group. At 24 months, PLLA mesh maintained the internal space just as well as that at 12 months, while the PLLA capsule collapsed and accumulated a large number of macrophages. The formed adipose tissue in the PLLA mesh group was maintained up to 24 months; however, those in two PLLA capsule groups decreased and showed no difference from the control group. In conclusion, the internal space of the PLLA mesh implant with CS was substituted for adipose tissue at 12 months and sustained the formed adipose tissue after 24 months. The PLLA mesh implant containing CS is a desirable bioabsorbable implant that can be replaced by autologous adipose tissue after implantation in vivo without using any growth factors or cells.
在乳腺癌患者中，不添加额外的生长因子或脂肪组织来源的基质细胞即可实现脂肪组织再生，这是因为存在复发和转移的可能性。我们报告称，一种含有胶原海绵（CS）的聚 L-乳酸（PLLA）网片植入物可在体内维持内部空间长达 12 个月，并替代脂肪组织。我们开发了一种 PLLA 胶囊，其维持内部空间的时间长于 PLLA 网片，并在兔模型中比较了植入 PLLA 网片加 CS 植入物与植入 PLLA 胶囊加或不加 CS 之间在植入后 12 个月和 24 个月时的脂肪组织形成情况。12 个月后，所有植入物均维持了内部空间，所有植入物组形成的脂肪组织均大于对照组。24 个月时，PLLA 网片维持内部空间的效果与 12 个月时相同，而 PLLA 胶囊则塌陷并积聚了大量巨噬细胞。PLLA 网片组形成的脂肪组织可维持至 24 个月，但两个 PLLA 胶囊组的脂肪组织减少，与对照组无差异。总之，CS 与 PLLA 网片植入物的内部空间在 12 个月时被脂肪组织替代，并在 24 个月后维持已形成的脂肪组织。含有 CS 的 PLLA 网片植入物是一种理想的可生物吸收植入物，可在体内植入后无需使用任何生长因子或细胞即可被自体脂肪组织替代。
