Hsa-circRNA-103809 通过 microRNA-1270/PLAG1 样锌指蛋白 2 轴促进肝癌发展。

Hsa-circRNA-103809 Promotes Hepatocellular Carcinoma Development via MicroRNA-1270/PLAG1 Like Zinc Finger 2 Axis.

机构信息

Department of General Surgery, Nanjing Drum Tower Hospital, No. 321 Zhongshan Road, Nanjing, 210000, Jiangsu, China.

Department of General Surgery, Zhongda Hospital, Clinical School of Southeast University, Nanjing, 210000, Jiangsu, China.

出版信息

Dig Dis Sci. 2021 May;66(5):1524-1532. doi: 10.1007/s10620-020-06416-x. Epub 2020 Jul 18.

DOI:10.1007/s10620-020-06416-x

PMID:32683589

Abstract

BACKGROUND

Hepatocellular carcinoma (HCC) is the second leading cause of cancer-related death in the worldwide. A great number of reports manifested that circular RNA hsa-circRNA-103809 (circRNA-103809) could work in several cancers.

AIMS

This study aimed to explore the function and mechanism of circRNA-103809 in HCC.

METHODS

Gene expressions were detected by quantitative real-time polymerase chain reaction. Colony formation, cell counting kit-8, transwell and wound healing assays were implemented to check the role of circRNA-103809 in HCC. Subcellular fractionation analysis was designed to figure out the cellular location of circRNA-103809. Luciferase reporter assay and RNA pull down assay were employed to verify the relationships among RNAs.

RESULTS

CircRNA-103809 was highly expressed in HCC cell lines. After interfering circRNA-103809, the proliferation, migration, invasion and epithelial-to-mesenchymal transition process were all hindered in HCC cells. Significantly, circRNA-103809 competed with PLAG1 like zinc finger 2 (PLAGL2) for binding with microRNA-1270 (miR-1270), which formulated a competing endogenous RNA network in HCC. Thereafter, we verified the tumor-facilitating effect of circRNA-103809/miR-1270/PLAGL2 axis on biological behaviors of HCC cells.

CONCLUSION

Hsa-circRNA-103809 promoted development of HCC via sequestering miR-1270 and up-regulating PLAGL2.

摘要

背景

肝细胞癌（HCC）是全球癌症相关死亡的第二大原因。大量报告表明，环状 RNA hsa-circRNA-103809（circRNA-103809）可以在几种癌症中发挥作用。

目的

本研究旨在探讨 circRNA-103809 在 HCC 中的功能和机制。

方法

通过实时定量聚合酶链反应检测基因表达。通过集落形成、细胞计数试剂盒-8、Transwell 和划痕愈合实验检查 circRNA-103809 在 HCC 中的作用。设计亚细胞分离分析以确定 circRNA-103809 的细胞内位置。使用荧光素酶报告基因检测和 RNA 下拉实验验证 RNA 之间的关系。

结果

circRNA-103809 在 HCC 细胞系中高表达。干扰 circRNA-103809 后，HCC 细胞的增殖、迁移、侵袭和上皮间质转化过程均受到抑制。重要的是，circRNA-103809 与 PLAG1 样锌指 2（PLAGL2）竞争结合 microRNA-1270（miR-1270），在 HCC 中构成了一个竞争性内源性 RNA 网络。此后，我们验证了 circRNA-103809/miR-1270/PLAGL2 轴对 HCC 细胞生物学行为的促进作用。