Luo Qing, Fu Biqi, Zhang Lu, Guo Yang, Huang Zikun, Li Junming
Department of Clinical Laboratory, The First Affiliated Hospital of Nanchang University, Nanchang, Jiangxi 330006, China.
Department of Rheumatology, The First Affiliated Hospital of Nanchang University, Nanchang, Jiangxi, China.
Dis Markers. 2020 Jun 25;2020:8193895. doi: 10.1155/2020/8193895. eCollection 2020.
Although it has been proved that the epigenetic modification of DNA and histones is involved in the pathogenesis of systemic lupus erythematosus (SLE), there is no study to explore whether the modification of N6-methyladenosine (m6A) in RNA is involved. In this study, the mRNA levels of m6A "writers" (, , and ), "erasers" ( and ), and "readers" () in peripheral blood were determined by quantitative reverse transcription-polymerase chain reaction (qRT-PCR). The results demonstrated that the mRNA levels of , , , , and in peripheral blood from SLE patients were significantly decreased. The levels of mRNA in SLE patients were associated with anti-dsDNA, antinucleosome, rash, and ulceration. Multivariate logistic regression analysis showed that the level of ALKBH5 mRNA in peripheral blood is a risk factor of SLE ( < 0.001). Moreover, our results suggested that there was a positive correlation between m6A"writers" ( and ), "erasers" ( and ), and "readers" () in SLE patients. This study suggests that the mRNA level of in peripheral blood may be involved in the pathogenesis of SLE.
虽然已经证明DNA和组蛋白的表观遗传修饰参与系统性红斑狼疮（SLE）的发病机制，但尚无研究探讨RNA中N6-甲基腺苷（m6A）修饰是否参与其中。在本研究中，通过定量逆转录-聚合酶链反应（qRT-PCR）测定外周血中m6A“书写器”（ 、 和 ）、“擦除器”（ 和 ）以及“阅读器”（ ）的mRNA水平。结果表明，SLE患者外周血中 、 、 、 和 的mRNA水平显著降低。SLE患者的 mRNA水平与抗双链DNA、抗核小体、皮疹和溃疡相关。多因素逻辑回归分析显示，外周血中ALKBH5 mRNA水平是SLE的危险因素（ < 0.001）。此外，我们的结果表明，SLE患者中m6A“书写器”（ 和 ）、“擦除器”（ 和 ）以及“阅读器”（ ）之间存在正相关。本研究提示外周血中 的mRNA水平可能参与SLE的发病机制。
