氧化应激标志物在两种胎盘功能障碍性疾病中存在差异：妊娠高血压和胎儿宫内生长受限。
Oxidative Stress Markers Differ in Two Placental Dysfunction Pathologies: Pregnancy-Induced Hypertension and Intrauterine Growth Restriction.
机构信息
1st Department of Obstetrics and Gynecology, Medical University of Warsaw, 1/3 Starynkiewicza Square, 02-015 Warsaw, Poland.
Department of Bioanalysis and Drugs Analysis, Faculty of Pharmacy, Medical University of Warsaw, 1 Banacha Street, 02-097 Warsaw, Poland.
出版信息
Oxid Med Cell Longev. 2020 Jun 30;2020:1323891. doi: 10.1155/2020/1323891. eCollection 2020.
AIM
Pregnancy-induced hypertension (PIH) and intrauterine growth restriction (IUGR) are both multisystemic disorders of pregnancy that cause perinatal morbidity and mortality. Recently, researchers focused on the role of oxidative stress (OS) as a pathophysiological mechanism in the development of these pathologies. The aim of this study was to compare OS in placental-related pathologies (PIH and IUGR) and uncomplicated pregnancies. We also investigated which salivary OS markers reflect systemic oxidative status and which only reflect the state of the oral cavity. . A total of 104 pregnant women ( = 104; 27 with PIH, 30 with IUGR, and 47 controls) were evaluated. Malondialdehyde (MDA), total antioxidant capacity (ORAC), aldehyde dehydrogenase (ALDH), and activity of glutathione peroxidase (GPx) and glutathione transferase (GST) in plasma/whole blood and/or saliva were analysed. Dietary nutrient intake was calculated using a Semiquantitative Food Frequency Questionnaire (SFFQ). Oral health was assessed to eliminate patients with bleeding, severe periodontitis, and other dental pathologies.
RESULTS
In the IUGR group, increased concentration of ORAC was observed both in saliva and plasma. Also, lower plasma levels of MDA in IUGR compared to the control group was detected. No sign of oxidative stress was confirmed in the PIH group. The examined groups did not differ regarding diet and markers of inflammation. ORAC in saliva was correlated with its level in plasma. No such correlations for MDA were observed. In the IUGR group, there were no differences in OS markers in plasma, but there was a lower ALDH level in the blood compared to the control group. It confirms OS occurrence in IUGR. In IUGR, a higher activity of salivary ALDH was probably due to worse oral health.
CONCLUSION
Oxidative stress differs between IUGR and PIH groups: the presence of oxidative stress was confirmed only in the IUGR group. Salivary ORAC can be used to estimate ORAC in plasma. The activity of salivary ALDH reflects the state of the oral cavity.
目的
妊娠高血压（PIH）和胎儿宫内生长受限（IUGR）都是妊娠多系统疾病，可导致围产期发病率和死亡率。最近，研究人员关注氧化应激（OS）作为这些病变发展的病理生理机制的作用。本研究旨在比较胎盘相关病变（PIH 和 IUGR）和非复杂妊娠中的 OS。我们还研究了哪些唾液 OS 标志物反映全身氧化状态，哪些仅反映口腔状态。共评估了 104 名孕妇（= 104；27 例 PIH，30 例 IUGR，47 例对照）。分析了血浆/全血和/或唾液中的丙二醛（MDA）、总抗氧化能力（ORAC）、醛脱氢酶（ALDH）以及谷胱甘肽过氧化物酶（GPx）和谷胱甘肽转移酶（GST）的活性。使用半定量食物频率问卷（SFFQ）计算膳食营养素摄入量。评估口腔健康以排除有出血、严重牙周炎和其他牙科疾病的患者。
结果
在 IUGR 组中，观察到唾液和血浆中 ORAC 的浓度均增加。此外，与对照组相比，IUGR 组血浆中 MDA 水平降低。在 PIH 组未发现氧化应激迹象。检查的各组在饮食和炎症标志物方面没有差异。唾液中的 ORAC 与其在血浆中的水平相关。未观察到 MDA 的相关性。在 IUGR 组中，血浆中的 OS 标志物没有差异，但与对照组相比，血液中的 ALDH 水平较低。这证实了 IUGR 中 OS 的发生。在 IUGR 中，唾液中更高的 ALDH 活性可能是由于口腔健康状况较差。
结论
IUGR 和 PIH 组之间的氧化应激不同：仅在 IUGR 组中证实存在氧化应激。唾液 ORAC 可用于估计血浆中的 ORAC。唾液 ALDH 的活性反映了口腔的状态。
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