Miramin-Mohammadi Akram, Javadi Amir, Eskandari Seyed Ebrahim, Mortazavi Hossein, Rostami Mahmoud Nateghi, Khamesipour Ali
Center for Research and Training in Skin Diseases and Leprosy, Tehran University of Medical Sciences, Tehran, Iran.
Department of Social Medicines, Qazvin University of Medical Sciences, Qazvin, Iran.
Iran J Microbiol. 2020 Jun;12(3):249-255.
The outcome of infection mainly depends upon the species which causes the disease and the generation of the type of host immune response, the healing process and protection in leishmaniasis depends upon induction of Th1 response. In this study, the Th1/Th2 cytokine profile in cutaneous leishmaniasis (CL) is evaluated.
This study was carried out in leishmaniasis clinic of CRTSDL, TUMS, during March 2018 to March 2019. Peripheral blood mononuclear cells (PBMC) of volunteers with active healing and non-healing lesion (s) of cutaneous leishmaniasis (CL), volunteers with and without history of CL were cultured and stimulated with Soluble antigen (SLA). The supernatants were collected and the levels of IFN-γ, IL-5 and IL-10 were titrated using ELISA method.
The results showed a significantly higher levels of IFN-γ in volunteers with active CL healing form (<0.005), history of CL (<0.005) than healthy volunteers. A significantly (<0.005) higher level of IFN-γ was seen in volunteers with active healing form of lesion than non-healing form. There was a significantly (<0.005) higher level of IL-10 in volunteers with a history of non-healing form and active non-healing form of CL. There was no significant difference in IL-5 production in PBMC of different groups.
IFN-γ production starts at early stage of cutaneous leishmaniasis and enhance during course of lesion healing, IFN-γ level is significantly higher in all patients compared to healthy volunteers, IFN-γ is significantly higher in patients with healing form than non-healing form of lesion.
感染的结果主要取决于引起疾病的物种以及宿主免疫反应类型的产生，利什曼病的愈合过程和保护取决于Th1反应的诱导。在本研究中，评估了皮肤利什曼病（CL）中的Th1/Th2细胞因子谱。
本研究于2018年3月至2019年3月在德黑兰医科大学CRTSDL的利什曼病诊所进行。对患有皮肤利什曼病（CL）活动性愈合和未愈合病变的志愿者、有和没有CL病史的志愿者的外周血单个核细胞（PBMC）进行培养，并用可溶性抗原（SLA）刺激。收集上清液，采用酶联免疫吸附测定（ELISA）法测定IFN-γ、IL-5和IL-10的水平。
结果显示，活动性CL愈合型志愿者（<0.005）、有CL病史的志愿者（<0.005）的IFN-γ水平显著高于健康志愿者。病变活动性愈合型志愿者的IFN-γ水平显著高于未愈合型（<0.005）。有CL未愈合型病史和活动性未愈合型的志愿者中IL-10水平显著更高（<0.005）。不同组PBMC中IL-5的产生无显著差异。
IFN-γ在皮肤利什曼病早期产生，并在病变愈合过程中升高，所有患者的IFN-γ水平均显著高于健康志愿者，愈合型病变患者的IFN-γ水平显著高于未愈合型。
