Kim Kwangwook, Ji Peng, Song Minho, Che Tung M, Bravo David, Pettigrew James E, Liu Yanhong
Department of Animal Science, University of California, Davis, CA USA.
Department of Nutrition, University of California, Davis, CA USA.
J Anim Sci Biotechnol. 2020 Jul 14;11:74. doi: 10.1186/s40104-020-00475-w. eCollection 2020.
Our previous study showed that 3 plant extracts enhanced the immune responses and growth efficiency of weaned pigs infected with porcine reproductive and respiratory syndrome virus (PRRSV), which is one of the most economically important disease in swine industry. However, each plant extract differently effected on growth efficiency and immune responses. Therefore, the objective of this study was conducted to characterize the effects and investigate the potential underlying mechanisms of 3 plant extracts on gene expression of alveolar macrophages in weaned pigs experimentally infected with PRRSV.
PRRSV infection altered ( < 0.05) the expression of 1,352 genes in pigs fed the control (CON; 755 up, 597 down). Compared with the infected CON, feeding capsicum (CAP), garlic botanical (GAR), or turmeric oleoresin (TUR) altered the expression of 46 genes (24 up, 22 down), 134 genes (59 up, 75 down), or 98 genes (55 up, 43 down) in alveolar macrophages of PRRSV-infected pigs, respectively. PRRSV infection up-regulated ( < 0.05) the expression of genes related to cell apoptosis, immune system process, and response to stimulus, but down-regulated ( < 0.05) the expression of genes involved in signaling transduction and innate immune response. Compared with the infected CON, feeding TUR or GAR reduced ( < 0.05) the expression of genes associated with antigen processing and presentation, feeding CAP up-regulated ( < 0.05) the expression of genes involved in antigen processing and presentation. Supplementation of CAP, GAR, or TUR also enhanced ( < 0.05) the expression of several genes related to amino acid metabolism, steroid hormone synthesis, or RNA degradation, respectively.
The results suggest that 3 plant extracts differently regulated the expression of genes in alveolar macrophages of PRRSV-infected pigs, especially altering genes involved in immunity.
我们之前的研究表明，3种植物提取物可增强感染猪繁殖与呼吸综合征病毒（PRRSV）的断奶仔猪的免疫反应和生长效率，PRRSV是养猪业中经济影响最为重大的疾病之一。然而，每种植物提取物对生长效率和免疫反应的影响各不相同。因此，本研究旨在表征3种植物提取物对实验感染PRRSV的断奶仔猪肺泡巨噬细胞基因表达的影响，并探究其潜在机制。
PRRSV感染改变了（P<0.05）对照日粮（CON）组猪1352个基因的表达（755个上调，597个下调）。与感染CON组相比，饲喂辣椒（CAP）、大蒜提取物（GAR）或姜黄树脂（TUR）分别改变了PRRSV感染猪肺泡巨噬细胞中46个基因（24个上调，22个下调）、134个基因（59个上调，75个下调）或98个基因（55个上调，43个下调）的表达。PRRSV感染上调了（P<0.05）与细胞凋亡、免疫系统过程和刺激反应相关基因的表达，但下调了（P<0.05）参与信号转导和固有免疫反应基因的表达。与感染CON组相比，饲喂TUR或GAR降低了（P<0.05）与抗原加工和呈递相关基因的表达，饲喂CAP上调了（P<0.05）参与抗原加工和呈递基因的表达。补充CAP、GAR或TUR还分别增强了（P<0.05）与氨基酸代谢、类固醇激素合成或RNA降解相关的几个基因的表达。
结果表明，3种植物提取物对PRRSV感染猪肺泡巨噬细胞中的基因表达有不同的调节作用，尤其是改变了与免疫相关的基因。
