• 文献检索
  • 文档翻译
  • 深度研究
  • 学术资讯
  • Suppr Zotero 插件Zotero 插件
  • 邀请有礼
  • 套餐&价格
  • 历史记录
应用&插件
Suppr Zotero 插件Zotero 插件浏览器插件Mac 客户端Windows 客户端微信小程序
定价
高级版会员购买积分包购买API积分包
服务
文献检索文档翻译深度研究API 文档MCP 服务
关于我们
关于 Suppr公司介绍联系我们用户协议隐私条款
关注我们

Suppr 超能文献

核心技术专利：CN118964589B侵权必究
粤ICP备2023148730 号-1Suppr @ 2026

文献检索

告别复杂PubMed语法，用中文像聊天一样搜索，搜遍4000万医学文献。AI智能推荐，让科研检索更轻松。

立即免费搜索

文件翻译

保留排版，准确专业，支持PDF/Word/PPT等文件格式，支持 12+语言互译。

免费翻译文档

深度研究

AI帮你快速写综述，25分钟生成高质量综述，智能提取关键信息，辅助科研写作。

立即免费体验

几丁质酶Chit33对不同几丁质分解材料的特异性使得能够生产具有抗氧化活性的未被探索的低聚壳聚糖。

-chitinase Chit33 specificity on different chitinolytic materials allows the production of unexplored chitooligosaccharides with antioxidant activity.

作者信息

Kidibule Peter Elias, Santos-Moriano Paloma, Plou Francisco Jose, Fernández-Lobato María

机构信息

Department of Molecular Biology, Centre for Molecular Biology Severo Ochoa (CSIC-UAM), University Autonomous from Madrid. Nicolás Cabrera, 1. Cantoblanco. 28049 Madrid, Spain.

Institute of Catalysis and Petrochemistry, CSIC. Marie Curie, 2. Cantoblanco, 28049 Madrid, Spain.

出版信息

Biotechnol Rep (Amst). 2020 Jul 4;27:e00500. doi: 10.1016/j.btre.2020.e00500. eCollection 2020 Sep.

DOI:10.1016/j.btre.2020.e00500
PMID:32685384
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC7355052/
Abstract

The biological activity of chitooligosaccharides (COS) has made them targets for industrial and medical sectors. In this work, chitinase Chit33 from CECT 2413 was expressed in GS115 to levels never achieved before (630 mg/L; 3.3 U/mL), without its biochemical characteristics being substantially affected. Chit33 produced a mixture of fully and partially acetylated COS from different chitin derivatives. HPAEC-PAD Chromatography and mass spectrometry analyses showed that (GlcNAc) and GlcN-(GlcNAc) were mainly produced from colloidal chitin and chitosan, respectively. COS in reaction mixtures were fragmented according to their size and their antioxidant activity analyzed by reducing power and free radical scavenging activity essays. The highest antioxidant activity was achieved with COS in the range of 0.5-2 and 2-10 kDa produced from colloidal chitin and chitosan, respectively, which gives biotechnological potential to both the chitin derivatives of 0.5-10 kDa and the biocatalyst producing them.

摘要

壳寡糖（COS）的生物活性使其成为工业和医疗领域的目标。在这项工作中，来自CECT 2413的几丁质酶Chit33在GS115中表达，达到了前所未有的水平（630mg/L；3.3U/mL），且其生化特性未受到实质性影响。Chit33从不同的几丁质衍生物中产生了完全乙酰化和部分乙酰化的COS混合物。高效阴离子交换色谱-脉冲安培检测（HPAEC-PAD）色谱和质谱分析表明，（GlcNAc）和GlcN-(GlcNAc)分别主要由胶体几丁质和壳聚糖产生。反应混合物中的COS根据其大小进行分离，并通过还原能力和自由基清除活性测定分析其抗氧化活性。分别由胶体几丁质和壳聚糖产生的0.5-2kDa和2-10kDa范围内的COS具有最高的抗氧化活性，这赋予了0.5-10kDa的几丁质衍生物及其生产生物催化剂生物技术潜力。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/be5a/7355052/7f1c33c00627/gr5.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/be5a/7355052/a7d712438388/fx1.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/be5a/7355052/cea90ed19b6d/gr1.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/be5a/7355052/d1ec9307628d/gr2.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/be5a/7355052/ac969718d4ad/gr3.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/be5a/7355052/2d935de13323/gr4.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/be5a/7355052/7f1c33c00627/gr5.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/be5a/7355052/a7d712438388/fx1.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/be5a/7355052/cea90ed19b6d/gr1.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/be5a/7355052/d1ec9307628d/gr2.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/be5a/7355052/ac969718d4ad/gr3.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/be5a/7355052/2d935de13323/gr4.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/be5a/7355052/7f1c33c00627/gr5.jpg

相似文献

1
-chitinase Chit33 specificity on different chitinolytic materials allows the production of unexplored chitooligosaccharides with antioxidant activity.几丁质酶Chit33对不同几丁质分解材料的特异性使得能够生产具有抗氧化活性的未被探索的低聚壳聚糖。
Biotechnol Rep (Amst). 2020 Jul 4;27:e00500. doi: 10.1016/j.btre.2020.e00500. eCollection 2020 Sep.
2
Use of chitin and chitosan to produce new chitooligosaccharides by chitinase Chit42: enzymatic activity and structural basis of protein specificity.利用几丁质酶 Chit42 将几丁质和壳聚糖转化为新型壳寡糖：酶的活性和蛋白特异性的结构基础。
Microb Cell Fact. 2018 Mar 22;17(1):47. doi: 10.1186/s12934-018-0895-x.
3
Chitinous material bioconversion by three new chitinases from the yeast Mestchnikowia pulcherrima.利用美丽毛霉中的三种新几丁质酶对几丁质物质进行生物转化。
Microb Cell Fact. 2024 Jan 20;23(1):31. doi: 10.1186/s12934-024-02300-9.
4
Enzymatic single-step preparation and antioxidant activity of hetero-chitooligosaccharides using non-pretreated housefly larvae powder.利用未经预处理的家蝇幼虫粉进行酶法一步制备杂合壳寡糖及抗氧化活性研究。
Carbohydr Polym. 2017 Sep 15;172:113-119. doi: 10.1016/j.carbpol.2017.05.037. Epub 2017 May 12.
5
A Recombinant Fungal Chitin Deacetylase Produces Fully Defined Chitosan Oligomers with Novel Patterns of Acetylation.一种重组真菌几丁质脱乙酰酶可产生具有新型乙酰化模式的完全确定的壳寡糖。
Appl Environ Microbiol. 2016 Oct 27;82(22):6645-6655. doi: 10.1128/AEM.01961-16. Print 2016 Nov 15.
6
Cloning, expression and biocharacterization of OfCht5, the chitinase from the insect Ostrinia furnacalis.昆虫奥司他韦的几丁质酶 OfCht5 的克隆、表达及生物特性分析。
Insect Sci. 2013 Apr;20(2):147-57. doi: 10.1111/j.1744-7917.2012.01512.x. Epub 2012 Jun 12.
7
Action pattern of Streptomyces griseus chitinase on partially N-acetylated chitosan.灰色链霉菌几丁质酶对部分N-乙酰化壳聚糖的作用模式
Agric Biol Chem. 1990 Dec;54(12):3191-9.
8
High level production of a Bacillus amlyoliquefaciens chitosanase in Pichia pastoris suitable for chitooligosaccharides preparation.在巴斯德毕赤酵母中高水平表达解几丁质酶适合制备壳寡糖。
Int J Biol Macromol. 2020 Apr 15;149:1034-1041. doi: 10.1016/j.ijbiomac.2020.02.001. Epub 2020 Feb 3.
9
Production of Structurally Defined Chito-Oligosaccharides with a Single -Acetylation at Their Reducing End Using a Newly Discovered Chitinase from .利用从……新发现的几丁质酶生产在其还原端具有单乙酰化的结构明确的壳寡糖。
J Agric Food Chem. 2021 Mar 24;69(11):3371-3379. doi: 10.1021/acs.jafc.0c06804. Epub 2021 Mar 10.
10
Characterization of the Specific Mode of Action of a Chitin Deacetylase and Separation of the Partially Acetylated Chitosan Oligosaccharides.壳聚糖脱乙酰酶特定作用模式的表征及部分乙酰化壳寡糖的分离。
Mar Drugs. 2019 Jan 22;17(2):74. doi: 10.3390/md17020074.

引用本文的文献

1
Boosting Biocatalytic Efficiency: Engineering of Chitinase Chit33 with Chitin and Cellulose Binding Domains for Sustainable Chitin Conversion.提高生物催化效率：构建具有几丁质和纤维素结合结构域的几丁质酶Chit33用于可持续的几丁质转化
J Agric Food Chem. 2025 May 7;73(18):11121-11131. doi: 10.1021/acs.jafc.4c10364. Epub 2025 Apr 25.
2
Insights into the transglucosylation activity of α-glucosidase from Schwanniomyces occidentalis.揭示产朊假丝酵母 α-葡萄糖苷酶的转葡糖苷活性。
Appl Microbiol Biotechnol. 2024 Aug 17;108(1):443. doi: 10.1007/s00253-024-13262-8.
3
Recombinant expression and characterization of the endochitinase Chit36-TA from Trichoderma asperellum in Komagataella phaffii for chitin degradation of black soldier fly exuviae.

本文引用的文献

1
Synthesis, characterization, and the antioxidant activity of the acetylated chitosan derivatives containing sulfonium salts.含𬭩盐的乙酰化壳聚糖衍生物的合成、表征及抗氧化活性。
Int J Biol Macromol. 2020 Jun 1;152:349-358. doi: 10.1016/j.ijbiomac.2020.02.177. Epub 2020 Feb 19.
2
Enzymatic Modification of Native Chitin and Conversion to Specialty Chemical Products.天然甲壳素的酶法修饰及特色化学品的转化。
Mar Drugs. 2020 Jan 30;18(2):93. doi: 10.3390/md18020093.
3
Heterologous expression and characterization of an antifungal chitinase (Chit46) from Trichoderma harzianum GIM 3.442 and its application in colloidal chitin conversion.
重组表达和鉴定里氏木霉内切几丁质酶 Chit36-TA 在毕赤酵母中的活性，用于黑水虻蜕皮的几丁质降解。
Bioprocess Biosyst Eng. 2024 Oct;47(10):1751-1766. doi: 10.1007/s00449-024-03067-4. Epub 2024 Aug 8.
4
A novel thermophilic chitinase directly mined from the marine metagenome using the deep learning tool Preoptem.一种利用深度学习工具Preoptem直接从海洋宏基因组中挖掘出的新型嗜热几丁质酶。
Bioresour Bioprocess. 2022 May 16;9(1):54. doi: 10.1186/s40643-022-00543-1.
5
and studies of the potential cytotoxic, antioxidant, and HMG CoA reductase inhibitory effects of chitin from Indonesia mangrove crab () shells.以及对来自印度尼西亚红树林蟹（）壳的几丁质的潜在细胞毒性、抗氧化和HMG CoA还原酶抑制作用的研究。 （括号内内容原文缺失具体物种名）
Saudi J Biol Sci. 2024 May;31(5):103964. doi: 10.1016/j.sjbs.2024.103964. Epub 2024 Feb 25.
6
Activity of a Recombinant Chitinase of the Ant on Different Forms of Chitin and Its Fungicidal Effect against .蚂蚁重组几丁质酶对不同形式几丁质的活性及其对……的杀真菌作用
Polymers (Basel). 2024 Feb 15;16(4):529. doi: 10.3390/polym16040529.
7
Chitinous material bioconversion by three new chitinases from the yeast Mestchnikowia pulcherrima.利用美丽毛霉中的三种新几丁质酶对几丁质物质进行生物转化。
Microb Cell Fact. 2024 Jan 20;23(1):31. doi: 10.1186/s12934-024-02300-9.
8
A Novel Porous Butyryl Chitin-Animal Derived Hydroxyapatite Composite Scaffold for Cranial Bone Defect Repair.一种新型多孔丁酰化壳聚糖-动物源性羟基磷灰石复合支架用于颅骨缺损修复。
Int J Mol Sci. 2023 May 10;24(10):8519. doi: 10.3390/ijms24108519.
9
Crude Enzyme Concentrate of Filamentous Fungus Hydrolyzed Chitosan to Obtain Oligomers of Different Sizes.丝状真菌粗酶浓缩物水解壳聚糖以获得不同大小的低聚物。
Polymers (Basel). 2023 Apr 27;15(9):2079. doi: 10.3390/polym15092079.
10
Structure-Function Insights into the Fungal -Chitinase Chit33 Depict its Mechanism on Chitinous Material.真菌 -几丁质酶 Chit33 的结构 -功能洞察揭示了其在几丁质材料上的作用机制。
Int J Mol Sci. 2022 Jul 9;23(14):7599. doi: 10.3390/ijms23147599.
哈茨木霉 GIM 3.442 抗菌几丁质酶（Chit46）的异源表达和特性及其在胶体几丁质转化中的应用。
Int J Biol Macromol. 2019 Aug 1;134:113-121. doi: 10.1016/j.ijbiomac.2019.04.177. Epub 2019 Apr 26.
4
Antifungal and Antioxidant Properties of Chitosan Polymers Obtained from Nontraditional Sources.非传统来源壳聚糖聚合物的抗真菌和抗氧化性能。
Mar Drugs. 2019 Apr 22;17(4):239. doi: 10.3390/md17040239.
5
Use of chitin and chitosan to produce new chitooligosaccharides by chitinase Chit42: enzymatic activity and structural basis of protein specificity.利用几丁质酶 Chit42 将几丁质和壳聚糖转化为新型壳寡糖：酶的活性和蛋白特异性的结构基础。
Microb Cell Fact. 2018 Mar 22;17(1):47. doi: 10.1186/s12934-018-0895-x.
6
Chitooligosaccharides and their biological activities: A comprehensive review.壳寡糖及其生物活性：全面综述。
Carbohydr Polym. 2018 Mar 15;184:243-259. doi: 10.1016/j.carbpol.2017.12.067. Epub 2017 Dec 28.
7
Effects of chitosan molecular weight on its antioxidant and antimutagenic properties.壳聚糖分子量对其抗氧化和抗突变性能的影响。
Carbohydr Polym. 2018 Feb 1;181:1026-1032. doi: 10.1016/j.carbpol.2017.11.047. Epub 2017 Nov 16.
8
Determination of the Deacetylation Degree of Chitooligosaccharides.壳寡糖脱乙酰度的测定。
Mar Drugs. 2017 Oct 25;15(11):332. doi: 10.3390/md15110332.
9
Radical-Scavenging Activity and Ferric Reducing Ability of Juniperus thurifera (L.), J. oxycedrus (L.), J. phoenicea (L.) and Tetraclinis articulata (L.).刺柏、刺叶石楠、地中海刺柏和龙柏的自由基清除活性及铁还原能力
Adv Pharmacol Sci. 2016;2016:6392656. doi: 10.1155/2016/6392656. Epub 2016 May 16.
10
Chitinases from Bacteria to Human: Properties, Applications, and Future Perspectives.从细菌到人类的几丁质酶：特性、应用及未来展望
Enzyme Res. 2015;2015:791907. doi: 10.1155/2015/791907. Epub 2015 Nov 19.