有丝分裂中纺锤体定位蛋白在胞质分裂中期的正确定位依赖于 IQGAP1。
The Correct Localization of Borealin in Midbody during Cytokinesis Depends on IQGAP1.
机构信息
State Key Laboratory of Genetic Engineering, Institute of Genetics, School of Life Sciences, Fudan University, Shanghai 200433, China.
Key laboratory of Medical Molecular Virology, Institute of Biomedical Sciences and Institute of Medical Microbiology, Shanghai Medical College, Fudan University, Shanghai 200032, China.
出版信息
Biomed Res Int. 2020 Jun 24;2020:6231697. doi: 10.1155/2020/6231697. eCollection 2020.
Borealin is a key component of chromosomal passenger complex, which is vital in cytokinesis. IQ domain-containing GTPase-activating protein 1 (IQGAP1) also participates in cytokinesis. The correlation between Borealin and IQGAP1 during cytokinesis is not yet clear. Here, we used mass spectrometry and endogenous coimmunoprecipitation experiments to investigate the interaction between IQGAP1 and Borealin. Results of the current study showed that Borealin interacted directly with IQGAP1 both and . Knockdown of IQGAP1 resulted in an abnormal location of Borealin in the midbody. Knocking down Borealin alone, IQGAP1 alone, or Borealin and IQGAP1 at the same time inhibited the completion of cytokinesis and formed multinucleated cells. Our results indicated that IQGAP1 interacts with Borealin during cytokinesis, and the correct localization of Borealin in the midbody during cytokinesis is determined by IQGAP1, and IQGAP1 may play an important role in regulating Borealin function in cytokinesis.
博雷琳是染色体乘客复合物的关键组成部分，对于胞质分裂至关重要。IQ 结构域包含的 GTP 酶激活蛋白 1（IQGAP1）也参与胞质分裂。博雷琳和 IQGAP1 在胞质分裂过程中的相关性尚不清楚。在这里，我们使用质谱和内源性共免疫沉淀实验来研究 IQGAP1 和博雷琳之间的相互作用。本研究结果表明，博雷琳与 IQGAP1 直接相互作用。敲低 IQGAP1 导致博雷琳在中体的位置异常。单独敲低博雷琳、IQGAP1 或同时敲低博雷琳和 IQGAP1 均抑制胞质分裂的完成，并形成多核细胞。我们的结果表明，IQGAP1 在胞质分裂过程中与博雷琳相互作用，IQGAP1 决定博雷琳在胞质分裂中中体的正确定位，IQGAP1 可能在调节博雷琳在胞质分裂中的功能方面发挥重要作用。
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