Department of Laboratory Medicine, The Affiliated People's Hospital of Fujian University of Traditional Chinese Medicine, Fuzhou, China.
Department of Laboratory Medicine, The First Affiliated Hospital of Fujian Medical University, Fuzhou, China.
J Clin Lab Anal. 2020 Nov;34(11):e23487. doi: 10.1002/jcla.23487. Epub 2020 Jul 19.
To analyze the association between positive urinary casts on microscopic examination and urinary microprotein concentration in the case of negative urinary protein test results. This study also investigated the diagnostic value of urinary microprotein examination.
A total of 949 samples that were analyzed with a UF-1000i Urine Analyzer and returned cast alarm results were categorized into two groups, a positive and negative group, according to qualitative urinary protein sulfosalicylic acid test results. Then, 54 samples with negative protein test results but positive cast results according to microscopic examination were selected as the study group; 60 normal people with healthy physical examination results were selected as the control group. Both groups underwent urinary microprotein tests, including urinary microalbumin (mAlb), α1-microglobulin (A1M), transferrin (TRU), and immunoglobulin G (IgG). T tests were used to evaluate mean differences between groups and chi-square tests were used to calculate ratio differences between groups.
(a) Microscopic examinations of the positive and negative protein groups revealed no statistically significant difference in cast detection rate (P = .421). (b) Among the 54 samples in the study group, 37 were found to have abnormal casts, while in the remaining 17 samples, only hyaline casts were detected. (c) The detection levels of mAlb, A1M, and IgG in the study group were significantly higher than the control group (P values < .05).
Urinary microprotein test should be included in the re-examination rules for routine tests for patients with negative protein results and positive casts under microscopic examination.
分析在尿蛋白检测结果阴性的情况下，尿沉渣阳性与尿微量蛋白浓度之间的关系。本研究还探讨了尿微量蛋白检测的诊断价值。
共 949 例 UF-1000i 尿液分析仪分析样本，根据定性尿蛋白磺基水杨酸法检测结果，将出现管型报警结果的标本分为两组，即阳性组和阴性组。然后，选取 54 例尿蛋白检测结果阴性但显微镜检查阳性的标本作为研究组；选取 60 例健康体检结果正常的人作为对照组。两组均进行尿微量蛋白检测，包括尿微量白蛋白（mAlb）、α1-微球蛋白（A1M）、转铁蛋白（TRU）和免疫球蛋白 G（IgG）。采用 t 检验评估组间均值差异，采用卡方检验计算组间比值差异。
（a）对阳性和阴性蛋白组进行显微镜检查，管型检出率无统计学差异（P=.421）。（b）在研究组的 54 例标本中，有 37 例发现异常管型，而在其余 17 例标本中，仅发现透明管型。（c）研究组的 mAlb、A1M 和 IgG 检测水平明显高于对照组（P 值均<.05）。
对于尿蛋白检测结果阴性但显微镜检查阳性的患者，应将尿微量蛋白检测纳入常规复查规则。
