Department of Anatomy, Faculty of Medicine, University of Malaya , Kuala Lumpur, Malaysia.
Department of Anatomy, College of Medical Sciences, Abubakar Tafawa Balewa University Bauchi , Bauchi, Nigeria.
Ultrastruct Pathol. 2020 Nov 20;44(4-6):359-371. doi: 10.1080/01913123.2020.1792597. Epub 2020 Jul 20.
Despite intense preclinical research focusing on developing potential strategies of mitigating spinal cord injury (SCI), SCI still results in permanent, debilitating symptoms for which there are currently no effective pharmacological interventions to improve the recovery of the fine ultrastructure of the spinal cord. is thought to have potential neuroprotective effects. We have previously demonstrated its protective potential on the lesioned corticospinal tracts and behavioral recovery. In this study, spirulina, known for its neuroprotective properties was used to further explore its protective effects on spinal cord gray matter ultrastructural. Twenty-four Sprague-Dawley rats were used and divided into sham group (laminectomy without SCI), control group (SCI without ), and group (SCI + 180 mg/kg ). All animals were anesthetized via intramuscular injection. A partial crush injury was induced at the level of T12. The rats were humanely sacrificed for 28 days postinjury for ultrastructural study. There were significant mean differences with respect to pairwise comparisons between the ultrastructural grading score of neuronal perikarya of control and the following injury at day 28, which correlates with the functional locomotor recovery at this timepoint in our previous study. The group supplemented with spirulina, thus, revealed a better improvement in the fine ultrastructure of the spinal cord gray matter when compared to the control group thereby suggesting neuroprotective potentials of spirulina in mitigating the effects of spinal cord injury and inducing functional recovery.
尽管有大量针对开发减轻脊髓损伤 (SCI) 的潜在策略的深入临床前研究，但 SCI 仍然导致永久性的、使人虚弱的症状，目前尚无有效的药物干预措施来改善脊髓的精细超微结构的恢复。 被认为具有潜在的神经保护作用。我们之前已经证明了它对损伤的皮质脊髓束和行为恢复的保护潜力。在这项研究中，螺旋藻以其神经保护特性而被用于进一步探索其对脊髓灰质超微结构的保护作用。使用 24 只 Sprague-Dawley 大鼠，并分为假手术组（无 SCI 的椎板切除术）、对照组（无 SCI 的 ）和 组（SCI + 180 mg/kg ）。所有动物均通过肌肉内注射麻醉。在 T12 水平诱导部分挤压伤。受伤后 28 天，对大鼠进行人道安乐死以进行超微结构研究。在神经元胞体的超微结构分级评分方面，控制组和 组在第 28 天的损伤之间存在显著的平均差异，这与我们之前的研究中此时点的功能运动恢复相关。与对照组相比，补充螺旋藻的 组显示出更好的脊髓灰质精细超微结构的改善，从而表明螺旋藻在减轻脊髓损伤的影响和诱导功能恢复方面具有神经保护潜力。
