Department of Clinical Biochemistry, School of Medicine, Zahedan University of Medical Sciences , Zahedan, Iran.
Cellular and Molecular Research Center, Resistant Tuberculosis Institute, Zahedan University of Medical Sciences , Zahedan, Iran.
Br J Biomed Sci. 2020 Oct;77(4):191-195. doi: 10.1080/09674845.2020.1769331. Epub 2020 Jul 20.
: miRNAs are small non-coding RNAs with potential roles in the complications of pregnancy. We hypothesised links between polymorphisms in miRNA-196a2 and miRNA-499 in maternal blood and the placentas of patients with preeclampsia. : The blood of 315 women with preeclampsia and 317 controls and the placentas of 103 PE and 133 healthy women were collected. The genotyping of both polymorphisms was performed by PCR-RFLP. : The maternal blood rs11614913 was unrelated to preeclampsia in genotype and allele models, but in placental tissue, the CT (odds ratio [95% CI] 0.5 [0.3-0.9, p = 0.018) and TT (0.4 [0.2-0.9] p = 0.033) genotypes alone and together (CT+TT v CC 0.5 [0.3-0.8] p = 0.009), and the T allele (0.6 [0.4-0.9], p = 0.019) were associated with lower risk of preeclampsia. The maternal blood rs3746444 CC genotype was more frequent in preeclampsia (2.2 [1.2-3.8] p = 0.008) and the recessive model (CC v TC+TT) was also significant (1.9 [1.1-3.3], p = 0.018), as was the C allele (1.4 [1.1-1.7] p = 0.014). In placental tissue, the increase in the frequency of the CC genotype was marginally significant (2.4 [1.0-5.8] p = 0.046). The maternal or placental miRNA-196a2 rs11614913 and miRNA-499 rs3746444 polymorphisms were unrelated to the severity of preeclampsia. : The placental but not maternal miRNA-196a2 rs11614913 variant could be a protective factor for preeclampsia predisposition in all models except the recessive model. The maternal/placental rs3746444 CC genotype was in association with higher preeclampsia risk.
miRNAs 是具有潜在作用的小非编码 RNA，可参与妊娠并发症。我们假设母体血液和子痫前期患者胎盘中小 miRNA-196a2 和 miRNA-499 多态性与子痫前期之间存在关联。
采集了 315 例子痫前期患者、317 例对照者的血液和 103 例 PE 患者及 133 例健康对照者的胎盘组织。采用 PCR-RFLP 对这两种多态性进行基因分型。
母体血液 rs11614913 与子痫前期的基因型和等位基因模型无关，但在胎盘组织中，CT（比值比 [95%CI] 0.5 [0.3-0.9，p = 0.018）和 TT（0.4 [0.2-0.9]，p = 0.033）基因型单独和共同（CT+TT v CC 0.5 [0.3-0.8]，p = 0.009），以及 T 等位基因（0.6 [0.4-0.9]，p = 0.019）与子痫前期风险降低相关。母体血液 rs3746444 CC 基因型在子痫前期中更为常见（2.2 [1.2-3.8]，p = 0.008），且隐性模型（CC v TC+TT）也有显著性差异（1.9 [1.1-3.3]，p = 0.018），C 等位基因（1.4 [1.1-1.7]，p = 0.014）也是如此。在胎盘组织中，CC 基因型的频率增加具有边缘显著性（2.4 [1.0-5.8]，p = 0.046）。母体或胎盘 miRNA-196a2 rs11614913 和 miRNA-499 rs3746444 多态性与子痫前期的严重程度无关。
除隐性模型外，胎盘而非母体的 miRNA-196a2 rs11614913 变体在所有模型中均可能是子痫前期易感性的保护因素。母体/胎盘 rs3746444 CC 基因型与较高的子痫前期风险相关。
