Institute of Biotechnology and Biochemical Engineering, Graz University of Technology, Graz 8010, Austria.
Institute of Food Science, Department of Food Science and Technology, University of Natural Resources and Life Sciences (BOKU), Vienna 1190, Austria.
J Agric Food Chem. 2020 Aug 12;68(32):8557-8567. doi: 10.1021/acs.jafc.0c02660. Epub 2020 Jul 31.
Short-chain cello-oligosaccharides (COS; degree of polymerization, DP ≤ 6) are promising water-soluble dietary fibers. An efficient approach to their bottom-up synthesis is from sucrose and glucose using glycoside phosphorylases. Here, we show the intensification and scale up (20 mL; gram scale) of COS production to 93 g/L product and in 82 mol % yield from sucrose (0.5 M). The COS were comprised of DP 3 (33 wt %), DP 4 (34 wt %), DP 5 (24 wt %), and DP 6 (9 wt %) and involved minimal loss (≤10 mol %) to insoluble fractions. After isolation (≥95% purity; ≥90% yield), the COS were examined for growth promotion of probiotic strains. Benchmarked against inulin, trans-galacto-oligosaccharides, and cellobiose, COS showed up to 4.1-fold stimulation of cell density for , subsp. , subsp. , and but were less efficient with sp. This study shows the COS as selectively functional carbohydrates with prebiotic potential and demonstrates their efficient enzymatic production.
短链纤维素寡糖（COS；聚合度 DP≤6）是很有前途的水溶性膳食纤维。使用糖苷磷酸化酶从蔗糖和葡萄糖中进行高效的从头合成是一种有效的方法。在这里，我们展示了 COS 生产的强化和放大（20 mL；克级规模），从蔗糖（0.5 M）中以 82 mol%的产率和 93 g/L 的产物达到 DP 3（33 wt%）、DP 4（34 wt%）、DP 5（24 wt%）和 DP 6（9 wt%）。涉及到的不溶性部分损失最小（≤10 mol%）。COS 经过分离（≥95%纯度；≥90%收率）后，用于考察其对益生菌菌株生长的促进作用。与菊粉、半乳糖-低聚糖和纤维二糖相比，COS 对 、 亚种、 亚种和 表现出高达 4.1 倍的细胞密度刺激作用，但对 种的作用效率较低。本研究表明，COS 是具有潜在益生元特性的选择性功能性碳水化合物，并展示了其高效的酶法生产。
