Konishi Atsumi, Eguchi Akira, Hosoi Fumiki, Omasa Kenji
Graduate School of Agricultural and Life Sciences, The University of Tokyo, Yayoi 1-1-1, Bunkyo-ku, Tokyo 113-8657, Japan.
Funct Plant Biol. 2009 Nov;36(11):874-879. doi: 10.1071/FP09108.
Spatio-temporal effects of herbicide including 3-(3,4 dichlorophenyl)-1,1-dimethylurea (DCMU) on a whole melon (Cucumis melo L.) plant were three-dimensionally monitored using combined range and chlorophyll a fluorescence imaging. The herbicide was treated to soil in a pot and the changes in chlorophyll a fluorescence images of the plant were captured over time. The time series of chlorophyll fluorescence images were combined with 3D polygon model of the whole plant taken by a high-resolution portable scanning lidar. From the produced 3D chlorophyll fluorescence model, it was observed that the increase of chlorophyll fluorescence appeared along veins of leaves and gradually expanded to mesophylls. In addition, it was found by detailed analysis of the images that the invisible herbicide injury on the mature leaves occurred earlier and more severely than on the young and old leaves. The distance from veins, whole leaf area and leaf inclination influenced the extent of the injury within the leaves. These results indicated difference in uptake of herbicide in the plant from soil depends on structural parameters of leaves and the microenvironments as well as leaf age. The findings showed that 3D monitoring using combined range and chlorophyll a fluorescence imaging can be utilised for understanding spatio-temporal changes of herbicide effects on a whole plant.
利用距离和叶绿素a荧光成像相结合的方法，对包括3-(3,4-二氯苯基)-1,1-二甲基脲（DCMU）在内的除草剂对甜瓜（Cucumis melo L.）整株植物的时空效应进行了三维监测。将除草剂施用于盆栽土壤中，并随时间捕捉植物叶绿素a荧光图像的变化。叶绿素荧光图像的时间序列与通过高分辨率便携式扫描激光雷达拍摄的整株植物的三维多边形模型相结合。从生成的三维叶绿素荧光模型中可以观察到，叶绿素荧光的增加沿着叶脉出现，并逐渐扩展到叶肉。此外，通过对图像的详细分析发现，成熟叶片上不可见的除草剂损伤比幼叶和老叶出现得更早、更严重。叶脉距离、全叶面积和叶片倾斜度影响叶片内的损伤程度。这些结果表明，植物从土壤中吸收除草剂的差异取决于叶片的结构参数、微环境以及叶龄。研究结果表明，利用距离和叶绿素a荧光成像相结合的三维监测方法可用于了解除草剂对整株植物影响的时空变化。
