Adams James E, Colombo Sergio L, Mason Catherine B, Ynalvez Ruby A, Tural Baran, Moroney James V
Department of Biological Sciences, Louisiana State University, Baton Rouge, LA 70803, USA.
Funct Plant Biol. 2005 Feb;32(1):55-66. doi: 10.1071/FP04119.
Photosynthetic microorganisms must acclimate to environmental conditions, such as low CO environments or high light intensities, which may lead to photo-oxidative stress. In an effort to understand how photosynthetic microorganisms acclimate to these conditions, Chlamydomonas reinhardtii was transformed using the Ble cassette, selected for Zeocin resistance and screened for colonies that showed poor growth at low CO levels. One of the insertional mutants obtained, named slc-230, was shown to have a Ble insert in the first exon of Hdh1, a novel, single copy gene. The predicted Hdh1 gene product has similarity to bacterial haloacid dehalogenase-like proteins, a protein family that includes phosphatases and epoxide hydrolases. In addition, Hdh1 is predicted to be localised to the chloroplast or mitochondria in C. reinhardtii. It was found that a genomic copy of wild type Hdh1 can complement slc-230.
光合微生物必须适应环境条件，如低二氧化碳环境或高光强度，这可能导致光氧化应激。为了了解光合微生物如何适应这些条件，使用Ble盒转化莱茵衣藻，选择对博来霉素有抗性的菌株，并筛选在低二氧化碳水平下生长不良的菌落。获得的一个插入突变体名为slc-230，显示在一个新的单拷贝基因Hdh1的第一个外显子中有一个Ble插入。预测的Hdh1基因产物与细菌卤代酸脱卤酶样蛋白相似，该蛋白家族包括磷酸酶和环氧化物水解酶。此外，预测Hdh1定位于莱茵衣藻的叶绿体或线粒体中。发现野生型Hdh1的基因组拷贝可以互补slc-230。
