Hossain Zahed, Mandal Abul Kalam Azad, Datta Subodh Kumar, Biswas Amal K
Botanic Garden and Floriculture, National Botanical Research Institute, Lucknow 226001, Uttar Pradesh, India.
Cytogenetics and Plant Breeding Laboratory, Botany Department, University of Kalyani, Kalyani 741235, West Bengal, India.
Funct Plant Biol. 2006 Feb;33(1):91-101. doi: 10.1071/FP05149.
A stable NaCl-tolerant mutant (R1) of Chrysanthemum morifolium Ramat has been developed by in vitro mutagenesis with gamma radiation (5 gray; Gy). Salt tolerance was evaluated by the capacity of the plant to maintain both flower quality and yield under NaCl stress. Enhanced salt tolerance of the R1 mutant was attributed to increased activities of reactive oxygen species (ROS)-scavenging enzymes, namely superoxide dismutase (SOD), monodehydroascorbate reductase (MDAR), dehydroascorbate reductase (DHAR) and glutathione reductase (GR), and to reduced membrane damage, higher relative water content (RWC), chlorophyll and carotenoids contents. RAPD analysis revealed two polymorphic bands (956 and 1093 bp) for the R1 mutant that might be considered as specific RAPD markers associated with salt tolerance. Better performance of the R1 progeny under identical salinity stress conditions, even in the second year, confirmed the genetic stability of the induced salt tolerance character. The R1 mutant developed by gamma ray treatment can be considered a salt-tolerant mutant showing all the positive characteristics of tolerance to NaCl stress.
通过γ射线（5戈瑞；Gy）体外诱变培育出了一种稳定的耐NaCl菊花（Chrysanthemum morifolium Ramat）突变体（R1）。通过植株在NaCl胁迫下维持花品质和产量的能力来评估耐盐性。R1突变体耐盐性增强归因于活性氧（ROS）清除酶，即超氧化物歧化酶（SOD）、单脱氢抗坏血酸还原酶（MDAR）、脱氢抗坏血酸还原酶（DHAR）和谷胱甘肽还原酶（GR）的活性增加，以及膜损伤减少、相对含水量（RWC）、叶绿素和类胡萝卜素含量更高。RAPD分析揭示了R1突变体的两条多态性条带（956和1093 bp），这可能被视为与耐盐性相关的特异性RAPD标记。即使在第二年，R1后代在相同盐度胁迫条件下的表现更好，证实了诱导的耐盐性状的遗传稳定性。通过γ射线处理培育出的R1突变体可被视为一个耐盐突变体，表现出对NaCl胁迫耐受的所有积极特征。
