Programa de Pós-Graduação em Ciências Farmacêuticas, Departamento de Análises Clínicas, Escola de Farmácia, Universidade Federal de Ouro Preto, Campus Morro do Cruzeiro, Ouro Preto, Minas Gerais, 35400-000, Brazil.
Programa de Pós-Graduação em Ciências Farmacêuticas, Departamento de Análises Clínicas, Escola de Farmácia, Universidade Federal de Ouro Preto, Campus Morro do Cruzeiro, Ouro Preto, Minas Gerais, 35400-000, Brazil.
Clin Nutr ESPEN. 2020 Aug;38:223-228. doi: 10.1016/j.clnesp.2020.04.007. Epub 2020 May 4.
BACKGROUND & AIMS: Diet and lifestyle play an important role in etiology of various tumors. Serum concentration of folate and vitamin B12may be associated with carcinogenesis since they are involved in DNA methylation and nucleotide synthesis. However, the role of these micronutrients on development of cervical cancer is still controversial. Thus, the aim of this study was to analyze the association of lower status of folate and vitamin B12 with the risk of pre-neoplastic cervical lesions.
Our sample group was divided in Control group (n = 120) - women with normal cytology, and Case groups (n = 57) - women presenting Atypical Squamous Cells of Undetermined Significance (ASC-US, n = 21), Low Grade Squamous Intraepithelial Lesion (LSIL; n = 16), and High-Grade lesions (n = 20). We obtained cervical samples for cytology analysis and HPV detection, and blood samples for evaluation of serum concentration of folate and vitamin B12.
No difference of serum folate was observed among Cases and Control groups. On the other hand, women with High-Grade lesions presented significant lower median concentration of vitamin B12 if compared to another groups. Then, we observed increased risk of High-Grade lesions among participants with low vitamin B12 levels was observed in relation to women that presented high levels of the micronutrient and from Control group [OR (95% CI): 2.09 (0.65-6.76), p = 0.216], ASC-US [OR (95% CI): 3.15 (0.82-12.08), p = 0.095], and LSIL [OR (95% CI): 3.10 (0.76-12.70), p = 0.116].
Low concentration of vitamin B12 was associated with an increased risk of High-Grade cervical lesions. Besides, we did not observe any difference of serum folate among women with normal cytology and women with pre-neoplastic cervical lesions.
饮食和生活方式在各种肿瘤的病因学中起着重要作用。叶酸和维生素 B12 的血清浓度可能与致癌作用有关，因为它们参与 DNA 甲基化和核苷酸合成。然而，这些微量营养素在宫颈癌发展中的作用仍存在争议。因此，本研究旨在分析叶酸和维生素 B12 状态较低与癌前宫颈病变风险的关系。
我们的样本组分为对照组（n=120）-细胞学正常的女性和病例组（n=57）-非典型鳞状细胞意义不明确（ASC-US，n=21）、低级别鳞状上皮内病变（LSIL；n=16）和高级别病变（n=20）。我们获得宫颈样本进行细胞学分析和 HPV 检测，并采集血液样本评估叶酸和维生素 B12 的血清浓度。
病例组和对照组之间的血清叶酸无差异。另一方面，与其他组相比，高级别病变的女性维生素 B12 中位数浓度明显较低。然后，我们观察到维生素 B12 水平较低的参与者中高级别病变的风险增加，与高水平维生素 B12 的女性和对照组相比[比值比（95%可信区间）：2.09（0.65-6.76），p=0.216]，ASC-US[比值比（95%可信区间）：3.15（0.82-12.08），p=0.095]和 LSIL[比值比（95%可信区间）：3.10（0.76-12.70），p=0.116]。
维生素 B12 浓度低与高级别宫颈病变的风险增加相关。此外，我们在细胞学正常的女性和癌前宫颈病变的女性之间没有观察到血清叶酸的任何差异。
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