Department of Stem Cell and Regenerative Biotechnology, Konkuk Institute of Technology, Konkuk University , Seoul, Korea.
Department of Bio-resources and Food Science, Konkuk University , Seoul, Korea.
Bioengineered. 2020 Dec;11(1):791-800. doi: 10.1080/21655979.2020.1794100.
Temozolomide (TMZ) is an alkylating chemotherapy agent used in the clinical treatment of glioblastoma multiforme (GBM) patients. Piperine (PIP) is a naturally occurring pungent nitrogenous substance present in the fruits of peppers. We investigated the anti-cancer efficacies of PIP alone and in combination with TMZ in GBM cellsusingparameters such as cell proliferation, cellular apoptosis,caspase-8/-9/-3 activities, cell cycle kinetics, wound-healing ability, and loss of mitochondrial membrane potential (MMP). Treatment with PIP and alow concentration of PIP-TMZ, inhibited cell growth, similar to TMZ.PIP-TMZ promoted apoptosis by activation of caspase-8/-9/-3, MMP loss, and inhibition of wound-healing motility. Reverse transcription polymerase chain reaction analysis showed significant inhibition of ( and expression upon treatment with a low concentration PIP-TMZ, suggesting an S to G1 arrest. Our findings provide insight into the apoptotic potential of the combination of a low concentration of PIP-TMZ, though further study will be needed for its validation.
替莫唑胺（TMZ）是一种烷化化疗药物，用于治疗多形性胶质母细胞瘤（GBM）患者。胡椒碱（PIP）是一种天然存在的刺激性含氮物质，存在于辣椒的果实中。我们研究了 PIP 单独使用和与 TMZ 联合使用在 GBM 细胞中的抗癌功效，使用了细胞增殖、细胞凋亡、半胱天冬酶-8/-9/-3 活性、细胞周期动力学、伤口愈合能力和线粒体膜电位（MMP）丧失等参数。与 TMZ 类似，PIP 和低浓度 PIP-TMZ 处理抑制细胞生长。PIP-TMZ 通过激活半胱天冬酶-8/-9/-3、MMP 丧失和抑制伤口愈合运动来促进细胞凋亡。逆转录聚合酶链反应分析显示，用低浓度 PIP-TMZ 处理后，（和表达显著抑制，表明 S 期到 G1 期阻滞。我们的研究结果为低浓度 PIP-TMZ 联合使用的凋亡潜力提供了深入了解，尽管需要进一步研究来验证。
