Ricotti Roberta, De Feudis Marilisa, Peri Caterina, Corazzari Marco, Genoni Giulia, Giordano Mara, Mancioppi Valentina, Agosti Emanuela, Bellone Simonetta, Prodam Flavia
Department of Health Sciences, University of Piemonte Orientale, Novara 28100, Italy.
Department of Translational Medicine, University of Piemonte Orientale, Novara 28100, Italy.
Int J Endocrinol. 2020 Jul 9;2020:6035138. doi: 10.1155/2020/6035138. eCollection 2020.
Haptoglobin (Hp) is a protein involved in the acute-phase reaction of inflammation. Humans have three major phenotypes (Hp1-1, Hp1-2, and Hp2-2). Several studies have shown altered Hp regulation in adults with obesity and metabolic alterations. The Hp2-2 phenotype is associated with a high cardiovascular risk. Our aim was to investigate if Hp levels and the Hp2-2 phenotype are associated with glucose metabolism in pediatric obesity.
We retrospectively studied 192 participants (92 males and 100 females), aged 4-18 years. Clinical and biochemical data were collected. The Hp phenotype (Hp1-1, Hp1-2, and Hp2-2) was identified through Western immunoblot.
Subjects carrying Hp1-1, Hp1-2, and Hp2-2 phenotypes were 13.6, 50.8, and 35.6%, respectively. Hp serum, fasting glucose, and insulin levels, as well as HOMA-IR, were similar among groups. Postload glucose and insulin levels (as insulin AUC) were progressively higher from the Hp1-1 to Hp2-2 phenotype.
To our knowledge, this is the first study on Hp phenotypes conducted in a pediatric population with obesity. We showed that the presence of Hp2 allele is associated with a worse response of glucose load in terms of both glucose and insulin levels. Thus, the Hp2-2 phenotype could predispose in pediatrics, at the same degree of obesity, to a worse glycemic and insulinemic compensation.
触珠蛋白（Hp）是一种参与炎症急性期反应的蛋白质。人类有三种主要表型（Hp1-1、Hp1-2和Hp2-2）。多项研究表明，肥胖和代谢改变的成年人中Hp调节发生了变化。Hp2-2表型与高心血管风险相关。我们的目的是研究Hp水平和Hp2-2表型是否与儿童肥胖中的葡萄糖代谢相关。
我们回顾性研究了192名年龄在4至18岁之间的参与者（92名男性和100名女性）。收集了临床和生化数据。通过Western免疫印迹法鉴定Hp表型（Hp1-1、Hp1-2和Hp2-2）。
携带Hp1-1、Hp1-2和Hp2-2表型的受试者分别为13.6%、50.8%和35.6%。各组之间的Hp血清、空腹血糖和胰岛素水平以及HOMA-IR相似。从Hp1-1到Hp2-2表型，负荷后血糖和胰岛素水平（以胰岛素AUC表示）逐渐升高。
据我们所知，这是首次在肥胖儿童人群中进行的关于Hp表型的研究。我们发现，从血糖和胰岛素水平来看，Hp2等位基因的存在与葡萄糖负荷的较差反应相关。因此，在肥胖程度相同的情况下，Hp2-2表型可能使儿童更容易出现较差的血糖和胰岛素代偿情况。
