Lustig H J, Nussenzweig V, Nussenzweig R S
J Immunol. 1977 Jul;119(1):210-6.
Immunoglobulin (Ig) is associated with erythrocyte membranes during infection of A/J mice with Plasmodium berghei. It was detected by agglutination of the cells with rabbit antibodies to mouse IgG and by a radioimmunoassay with 125I-labelled rabbit antibodies to mouse IgG. As shown by the degree of agglutination and of binding of radiolabeled antibodies to the erythrocyte membranes, the amount of Ig increased with time after infection and paralleled parasitemia and reticulocytosis. The erythrocyte-associated immunoglobulins are mainly IgG but IgM was also present on the cells of some mice. A large proportion of the Ig could be eluted at 37 degrees C and was analyzed by immunoprecipitation and acrylamide gel electrophoresis. A radioautographic study with 125I-labeled anti-mouse IgG revealed that both parasitized and nonparasitized reticulocytes of infected mice had much larger amounts of membrane-bound immunoglobulin than did mature, nonparasitized erythrocytes. The nature of the bonds between the Ig and the surface membrane of the reticulocytes is not known. The Ig could be part of immune complexes nonspecifically bound to the cell surface. However, since we have not detected Fc or C3d receptors on reticulocytes, it is possible that the Ig constitutes autoantibodies against reticulocytes or antibodies against parasitic antigens present on the cell membrane.
在用伯氏疟原虫感染A/J小鼠的过程中，免疫球蛋白（Ig）与红细胞膜相关。通过用兔抗小鼠IgG抗体使细胞凝集以及用125I标记的兔抗小鼠IgG进行放射免疫测定来检测它。正如放射性标记抗体与红细胞膜的凝集程度和结合程度所示，感染后Ig的量随时间增加，与寄生虫血症和网织红细胞增多平行。与红细胞相关的免疫球蛋白主要是IgG，但在一些小鼠的细胞上也存在IgM。大部分Ig可在37℃洗脱，并通过免疫沉淀和丙烯酰胺凝胶电泳进行分析。用125I标记的抗小鼠IgG进行的放射自显影研究表明，感染小鼠的寄生和未寄生网织红细胞比成熟的、未寄生的红细胞具有更多的膜结合免疫球蛋白。Ig与网织红细胞表面膜之间结合的性质尚不清楚。Ig可能是非特异性结合到细胞表面的免疫复合物的一部分。然而，由于我们在网织红细胞上未检测到Fc或C3d受体，因此Ig有可能构成针对网织红细胞的自身抗体或针对细胞膜上存在的寄生虫抗原的抗体。
