Wofsy L, McDevitt H O, Henry C
J Immunol. 1977 Jul;119(1):61-4.
The capping of Ia antigens does not induce redistribution of Fc receptors (FcR) on B lymphocytes. This rules out the possibility of a unidirectional association between Ia and FcR such as has been reported to link Ig and FcR. Ia-capping was achieved with hapten-sandwich antibodies devoid of Fc regions: hapten-conjugated Fab anti-Ia followed by (Fab')2 anti-hapten antibody. Three different immune complex systems were used to label FcR. With fluorescent double labeling, Ia and FcR were readily distinguished. The independent labeling and surface mobility of Ia and FcR are considered in connection with reports of the inhibition of FcR by anti-Ia antibodies.
Ia抗原的封帽现象不会诱导B淋巴细胞上Fc受体（FcR）的重新分布。这排除了Ia与FcR之间存在单向关联的可能性，就如同据报道存在于Ig与FcR之间的那种关联。通过不含Fc区域的半抗原夹心抗体实现了Ia封帽：半抗原偶联的抗Ia Fab抗体，随后是（Fab'）2抗半抗原抗体。使用了三种不同的免疫复合物系统来标记FcR。通过荧光双重标记，Ia和FcR很容易区分。结合抗Ia抗体对FcR抑制作用的报道，对Ia和FcR的独立标记及表面流动性进行了探讨。
