Novaes-Ledieu M, Martínez Cobo J A, García Mendoza C
Centro de Investigaciones Biológicas, C.S.I.C., Velázquez, Madrid, Spain.
Microbiologia. 1987 Feb;3(1):13-23.
The chemical composition of fractions extracted from the mycelial walls of Agaricus bisporus was studied. Attempts were made to locate these materials "in situ" by comparing electron micrographs of shadowed and sectioned cell walls. There are at least two chemically distinct species of carbohydrate polymers: one yielding mainly glucose with lower amounts of mannose, galactose and xylose and amino acids, and the other yielding chiefly N-acetylglucosamine with some glucose and amino acids. It was shown that none of these polymers constituted a completely physically distinct layer, but there do seem to be two regions at the outer and inner surfaces with apparently different properties. Materials which react with MnO4K to produce an electron-dense material are located throughout the wall, but tend to be concentrated in the outer and inner regions. It was shown that fibers constitute one important fraction of the original wall material.
对双孢蘑菇菌丝体壁提取物的化学成分进行了研究。通过比较经投影和切片处理的细胞壁电子显微镜照片，试图在“原位”定位这些物质。至少有两种化学性质不同的碳水化合物聚合物：一种主要产生葡萄糖，还有少量甘露糖、半乳糖、木糖和氨基酸；另一种主要产生N-乙酰葡糖胺，还有一些葡萄糖和氨基酸。结果表明，这些聚合物均未构成完全物理上不同的层，但在外表面和内表面似乎确实存在两个性质明显不同的区域。与高锰酸钾反应产生电子致密物质的材料遍布整个细胞壁，但往往集中在外层和内层区域。结果表明，纤维是原始细胞壁材料的一个重要组成部分。
