Department of Pharmacology & Therapeutics, University of Florida, 1200 Newell Dr., Gainesville, FL 32610, USA; Department of Neurosciences, Case Western Reserve University, 2109 Adelbert Rd., Cleveland, OH 44106, USA.
Department of Pharmacology & Therapeutics, University of Florida, 1200 Newell Dr., Gainesville, FL 32610, USA.
Cell Rep. 2020 Jul 21;32(3):107919. doi: 10.1016/j.celrep.2020.107919.
Odors are well known to elicit strong emotional and behavioral responses that become strengthened throughout learning, yet the specific cellular systems involved in odor learning and the direct influence of these on behavior are unclear. Here, we investigate the representation of odor-reward associations within two areas recipient of dense olfactory input, the posterior piriform cortex (pPCX) and the olfactory tubercle (OT), using electrophysiological recordings from mice engaged in reward-based learning. Neurons in both regions represent conditioned odors and do so with similar information content, yet the proportion of neurons recruited by conditioned rewarded odors and the magnitudes and durations of their responses are greater in the OT. Using fiber photometry, we find that OT D1-type dopamine-receptor-expressing neurons flexibly represent odors based on reward associations, and using optogenetics, we show that these neurons influence behavioral engagement. These findings contribute to a model whereby OT D1 neurons support odor-guided motivated behaviors.
气味众所周知会引起强烈的情绪和行为反应，这些反应会在学习过程中得到加强，但参与气味学习的特定细胞系统以及这些系统对行为的直接影响尚不清楚。在这里，我们使用参与基于奖励的学习的小鼠的电生理记录，研究了两个密集接受嗅觉输入的区域（后梨状皮层[pPCX]和嗅结节[OT]）中气味-奖励关联的表示。这两个区域中的神经元都代表条件化的气味，并且具有相似的信息含量，但在 OT 中，由条件奖励气味募集的神经元的比例以及它们的反应幅度和持续时间更大。使用光纤光度测定法，我们发现 OT D1 型多巴胺受体表达神经元可根据奖励关联灵活地表示气味，并且使用光遗传学，我们表明这些神经元会影响行为参与。这些发现有助于建立一个模型，即 OT D1 神经元支持气味引导的动机行为。
