Janelia Research Campus, Howard Hughes Medical Institute, Ashburn, VA 20147, USA; Graduate School, University of Arkansas for Medical Sciences, Little Rock, AR 72205, USA; College of Medicine, University of Arkansas for Medical Sciences, Little Rock, AR 72205, USA.
Janelia Research Campus, Howard Hughes Medical Institute, Ashburn, VA 20147, USA.
Cell Chem Biol. 2020 Aug 20;27(8):1063-1072.e7. doi: 10.1016/j.chembiol.2020.07.001. Epub 2020 Jul 21.
Light-mediated chemical reactions are powerful methods for manipulating and interrogating biological systems. Photosensitizers, compounds that generate reactive oxygen species upon excitation with light, can be utilized for numerous biological experiments, but the repertoire of bioavailable photosensitizers is limited. Here, we describe the synthesis, characterization, and utility of two photosensitizers based upon the widely used rhodamine scaffold and demonstrate their efficacy for chromophore-assisted light inactivation, cell ablation in culture and in vivo, and photopolymerization of diaminobenzidine for electron microscopy. These chemical tools will facilitate a broad range of applications spanning from targeted destruction of proteins to high-resolution imaging.
光介导的化学反应是用于操纵和研究生物系统的强大方法。光激发后能产生活性氧物质的光敏剂可用于许多生物学实验，但可用的光敏剂种类有限。在这里，我们描述了两种基于广泛使用的罗丹明支架的光敏剂的合成、表征和用途，并证明了它们在发色团辅助光失活、培养物中和体内的细胞消融以及二氨基联苯胺的光聚合用于电子显微镜中的功效。这些化学工具将促进广泛的应用，从靶向蛋白质破坏到高分辨率成像。
