Jonsdottir-Buch Sandra M, Gunnarsdottir Kristbjorg, Sigurjonsson Olafur E
The Blood Bank, Landspitali-The National University Hospital of Iceland, Snorrabraut 60, 101 Reykjavik, Iceland.
Faculty of Medicine, University of Iceland, Vatnsmyrarvegur 16, 101 Reykjavik, Iceland.
Bioengineering (Basel). 2020 Jul 20;7(3):75. doi: 10.3390/bioengineering7030075.
Human embryonic stem cell-derived mesenchymal progenitor (hES-MP) cells are mesenchymal-like cells, derived from human embryonic stem cells without the aid of feeder cells. They have been suggested as a potential alternative to mesenchymal stromal cells (MSCs) in regenerative medicine due to their mesenchymal-like proliferation and differentiation characteristics. Cells and cell products intended for regenerative medicine in humans should be derived, expanded and differentiated using conditions free of animal-derived products to minimize risk of animal-transmitted disease and immune reactions to foreign proteins. Human platelets are rich in growth factors needed for cell culture and have been used successfully as an animal serum replacement for MSC expansion and differentiation. In this study, we compared the proliferation of hES-MP cells and MSCs; the hES-MP cell growth was sustained for longer than that of MSCs. Growth factors, gene expression, and surface marker expression in hES-MP cells cultured with either human platelet lysate (hPL) or fetal bovine serum (FBS) supplementation were compared, along with differentiation to osteogenic and chondrogenic lineages. Despite some differences between hES-MP cells grown in hPL- and FBS-supplemented media, hPL was found to be a suitable replacement for FBS. In this paper, we demonstrate for the first time that hES-MP cells can be grown using platelet lysates from expired platelet concentrates (hPL).
人胚胎干细胞来源的间充质祖细胞（hES-MP）是一种间充质样细胞，来源于人胚胎干细胞，无需饲养层细胞。由于其间充质样的增殖和分化特性，它们被认为是再生医学中间充质基质细胞（MSC）的一种潜在替代物。用于人类再生医学的细胞和细胞产品应在无动物源性产品的条件下进行衍生、扩增和分化，以尽量减少动物传播疾病的风险和对外源蛋白质的免疫反应。人血小板富含细胞培养所需的生长因子，并已成功用作MSC扩增和分化的动物血清替代品。在本研究中，我们比较了hES-MP细胞和MSC的增殖情况；hES-MP细胞的生长持续时间比MSC更长。比较了用人血小板裂解物（hPL）或胎牛血清（FBS）补充培养的hES-MP细胞中的生长因子、基因表达和表面标志物表达，以及向成骨和成软骨谱系的分化情况。尽管在补充hPL和FBS的培养基中生长的hES-MP细胞之间存在一些差异，但发现hPL是FBS的合适替代品。在本文中，我们首次证明hES-MP细胞可以使用过期血小板浓缩物中的血小板裂解物（hPL）进行培养。
