Helmholtz-Institute for Biomedical Engineering, Stem Cell Biology and Cellular Engineering, RWTH Aachen University Medical School, Aachen, 52074, Germany.
Institute for Biomedical Technology - Cell Biology, RWTH Aachen University Medical School, Aachen, 52074, Germany.
Sci Rep. 2017 Jul 11;7(1):5132. doi: 10.1038/s41598-017-05207-1.
Culture medium of mesenchymal stromal cells (MSCs) is usually supplemented with either human platelet lysate (HPL) or fetal calf serum (FCS). Many studies have demonstrated that proliferation and cellular morphology are affected by these supplements - it is therefore important to determine if they favor outgrowth of different subpopulations and thereby impact on the heterogeneous composition of MSCs. We have isolated and expanded human bone marrow-derived MSCs in parallel with HPL or FCS and demonstrated that HPL significantly increases proliferation and leads to dramatic differences in cellular morphology. Remarkably, global DNA-methylation profiles did not reveal any significant differences. Even at the transcriptomic level, there were only moderate changes in pairwise comparison. Furthermore, the effects on proliferation, cytoskeletal organization, and focal adhesions were reversible by interchanging to opposite culture conditions. These results indicate that cultivation of MSCs with HPL or FCS has no systematic bias for specific cell types.
间充质基质细胞（MSCs）的培养基通常添加人血小板裂解物（HPL）或胎牛血清（FCS）。许多研究表明，这些补充物会影响细胞增殖和形态 - 因此，确定它们是否有利于不同亚群的生长，从而影响 MSCs 的异质性组成非常重要。我们已经平行地使用 HPL 或 FCS 分离和扩增了人骨髓来源的 MSCs，并证明 HPL 可显著增加增殖，并导致细胞形态的显著差异。值得注意的是，全基因组 DNA 甲基化谱没有显示出任何显着差异。即使在转录组水平上，两两比较也只有适度的变化。此外，通过相互交换相反的培养条件，增殖、细胞骨架组织和焦点粘连的影响是可逆的。这些结果表明，用 HPL 或 FCS 培养 MSC 不会对特定细胞类型产生系统偏差。
