denovoMATRIX GmbH, Tatzberg 47, Dresden, 01307, Germany.
University Hospital Carl Gustav Carus der Technischen Universität Dresden, Medizinische Klinik und Poliklinik 1, Fetscherstraße 74, Dresden, 01307, Germany.
Adv Biosyst. 2020 Aug;4(8):e2000008. doi: 10.1002/adbi.202000008. Epub 2020 Jul 23.
Human mesenchymal stromal cells (hMSCs) have enormous potential for the treatment of various inflammatory and degenerative diseases. Their manufacturing for cell-based therapies requires extensive ex vivo expansion and optimal growth conditions. To support cell adhesion, spreading, and growth in serum-free culture conditions, the applied plasticware needs to be functionalized with essential biochemical cues. By employing a recently developed screening tool, a chemically defined functional matrix composed of dextran sulfate and a bone-related extracellular matrix peptide is identified, which supports long-term culture of bone marrow-derived hMSCs in serum-free culture conditions. Cells grown under these conditions display rapid proliferation and high viability while maintaining their differentiation and immunomodulatory capacity, characteristic cell morphology, expression of hMSC-specific surface antigens as well as important markers of stemness and differentiation potential. The chemically defined, serum-free culture environment enables reliable and reproducible expansion of hMSCs important for cell based-therapies, drug screening, and disease modeling.
人骨髓间充质干细胞（hMSCs）在治疗各种炎症和退行性疾病方面具有巨大的潜力。为了进行基于细胞的治疗，需要对其进行大量的体外扩增和优化生长条件。为了支持无血清培养条件下细胞的黏附、铺展和生长，应用的塑料器皿需要用基本的生化线索进行功能化。通过使用最近开发的筛选工具，确定了一种由硫酸葡聚糖和与骨相关的细胞外基质肽组成的化学定义功能基质，该基质支持骨髓来源的 hMSCs 在无血清培养条件下的长期培养。在这些条件下生长的细胞表现出快速增殖和高活力，同时保持其分化和免疫调节能力、典型的细胞形态、hMSC 特异性表面抗原的表达以及干性和分化潜能的重要标志物。这种化学定义的、无血清的培养环境能够可靠且可重复地扩增 hMSCs，这对于细胞治疗、药物筛选和疾病建模非常重要。
