Beyer Alina, Dalton Marly, Doll Katharina, Winkel Andreas, Stumpp Nico S, Stiesch Meike
Oral Health Prev Dent. 2020 Jul 24;18(3):625-632. doi: 10.3290/j.ohpd.a44938.
Currently, the prevention of periodontal diseases focuses on mechanical removal of pathogenic biofilms combined with oral antiseptics as supportive chemical antibacterial control. Due to the risk of resistance development and side effects of existing antiseptics, the interest in alternative medicine with naturopathic treatment modalities is growing in dentistry. In the present study, the antibacterial effect of the naturopathic oral care product Repha OS and some of its derivatives, based on medicinal plant extracts and essential oils, with a specific focus on added sweeteners, was investigated on periodontal pathogenic and halitosis-associated bacteria.
The antibacterial efficacy was investigated by agar dilution assay. The minimum inhibitory concentration (MIC) for the bacterial species Aggregatibacter actinomycetemcomitans, Fusobacterium nucleatum, Porphyromonas gingivalis, Prevotella intermedia and Solobacterium moorei was determined.
A concentration-dependent antibacterial effect on oral bacterial species by Repha OS and its derivatives was demonstrated. For the original product, the maximum MIC was 10% of the calculated test solution concentration in agar for all examined bacterial species. The removal of essential oils reduced the antibacterial efficacy, whereas the displacement or replacement of sweeteners had almost no effect.
In addition to other individual effects of the ingredients, the results of this study show that an antibacterial effect of the naturopathic oral care product on the tested oral bacterial species was achieved in vitro. In vivo, the combination of this antibacterial effect with other properties of the various ingredients may be interesting for a holistic approach in preventive dentistry.
目前，牙周疾病的预防主要集中在机械清除致病生物膜，并结合使用口腔抗菌剂作为辅助化学抗菌控制手段。由于现有抗菌剂存在产生耐药性的风险和副作用，牙科领域对采用自然疗法治疗方式的替代医学的兴趣日益浓厚。在本研究中，对基于药用植物提取物和精油的自然疗法口腔护理产品Repha OS及其一些衍生物的抗菌效果进行了研究，特别关注了添加的甜味剂对牙周病原菌和与口臭相关细菌的影响。
通过琼脂稀释法研究抗菌效果。测定了伴放线聚集杆菌、具核梭杆菌、牙龈卟啉单胞菌、中间普氏菌和穆尔氏栖组织杆菌等细菌的最低抑菌浓度（MIC）。
Repha OS及其衍生物对口腔细菌呈现出浓度依赖性抗菌作用。对于原始产品，所有检测细菌的最大MIC为琼脂中计算出的测试溶液浓度的10%。去除精油会降低抗菌效果，而甜味剂的置换或替代几乎没有影响。
除了成分的其他个体效应外，本研究结果表明，该自然疗法口腔护理产品在体外对测试的口腔细菌具有抗菌作用。在体内，这种抗菌作用与各种成分的其他特性相结合，对于预防牙科的整体方法可能具有重要意义。
