Department of Food Processing, Mugla Sitki Kocman University, Mugla, Turkey.
Department of Biology, Mugla Sitki Kocman University, Mugla, Turkey.
Biotechnol Appl Biochem. 2021 Aug;68(4):789-800. doi: 10.1002/bab.1991. Epub 2020 Aug 1.
In this study, a novel pH-sensitive hydrogel beads that is based on gelatin/sodium alginate/chitosan (GEL/SA/CS) loaded with propolis ethanolic extracts (PE) were synthesized. The swelling behavior of GEL/SA/CS hydrogel beads was studied in different pH solutions and compared with unloaded CS (GEL/SA) hydrogel beads. The in vitro release studies have been revealed using four different pH (1.3, 5.0, 6.0, and 6.8), a saliva environment (pH 6.8), a simulated gastric fluid (SGF) (pH 1.3), and a simulated intestinal fluid (SIF) (pH 6.8) to simulate the physiological conditions in gastrointestinal (GI) tract. Propolis-loaded hydrogel beads were found to be stable at pH 1.3, 5.0, 6.0, simulated saliva, SGF, and SIF mediums, whereas the beads lose their stability at pH 6.8 buffer solution. Tested microorganisms displayed greater sensitivity to PE-loaded hydrogel beads compared with pure propolis. Contrary to antimicrobial activity results, antibiofilm activity results of PE-loaded GEL/SA and GEL/SA/CS hydrogel beads were found at low levels. According to the obtained results, the propolis-loaded GEL/SA/CS hydrogel beads synthesized within this study can be used in the treatment of GI tract diseases such as oral mucositis, gastric ulcer, ulcerative colitis, and GI cancer, as controlled releasing carriers of propolis.
在这项研究中，合成了一种基于明胶/海藻酸钠/壳聚糖（GEL/SA/CS）负载蜂胶乙醇提取物（PE）的新型 pH 敏感水凝胶珠。研究了 GEL/SA/CS 水凝胶珠在不同 pH 溶液中的溶胀行为，并与未负载 CS（GEL/SA）水凝胶珠进行了比较。使用四种不同的 pH（1.3、5.0、6.0 和 6.8）、唾液环境（pH 6.8）、模拟胃液（SGF）（pH 1.3）和模拟肠液（SIF）（pH 6.8）进行了体外释放研究，以模拟胃肠道（GI）中的生理条件。蜂胶负载水凝胶珠在 pH 1.3、5.0、6.0、模拟唾液、SGF 和 SIF 介质中稳定，而在 pH 6.8 缓冲溶液中则失去稳定性。与纯蜂胶相比，测试的微生物对负载 PE 的水凝胶珠显示出更高的敏感性。与抗菌活性结果相反，负载 PE 的 GEL/SA 和 GEL/SA/CS 水凝胶珠的抗生物膜活性结果处于低水平。根据获得的结果，本研究中合成的负载蜂胶的 GEL/SA/CS 水凝胶珠可用作治疗 GI 道疾病的控释载体，如口腔粘膜炎、胃溃疡、溃疡性结肠炎和 GI 癌。
