Chapman R E, Hardy M H
Division of Animal Production, CSIRO, Blacktown, N.S.W.
Aust J Biol Sci. 1988;41(2):261-8. doi: 10.1071/bi9880261.
Twice daily intradermal (ID) injections of mouse epidermal growth factor (mEGF) in sterile saline for 1-4 days into delineated areas of skin of Merino sheep produced dose-dependent changes in wool follicles and fibres, ranging from slight reduction in follicle bulb size and transient disturbance of cuticle formation on some fibres to the induction of catagen of follicles and shedding of fibres with distorted, tapered ends. Regeneration of follicles commenced by day 7. By contrast, ID injections of saline did not affect follicle activity. The epidermis became thicker and more parakeratotic after multiple injections of mEGF than after injection of saline, but was almost normal again by day 14. Persistent small increases in sebaceous gland size, additional to those induced by ID injections of saline, and delayed small increases in sweat gland size also occurred after multiple injections of mEGF. Daily topical applications of mEGF in 50% (v/v) aqueous propylene glycol 5 days each week for 4 weeks did not affect wool growth or the follicles and other skin components. The only effect observed, due to application of the aqueous propylene glycol, was an increase in the number of layers of cornified cells in the stratum corneum of the epidermis, with the cells arranged in clearly discernible stacks. The effects produced by ID injections of mEGF indicate that mEGF acts directly on the pilosebaceous and epidermal components of skin.
将小鼠表皮生长因子（mEGF）以无菌生理盐水溶解，每日两次皮内（ID）注射到美利奴绵羊皮肤的划定区域，持续1 - 4天，结果发现毛囊和纤维出现剂量依赖性变化，从毛囊球部大小略有减小以及部分纤维角质层形成的短暂紊乱，到诱导毛囊进入退行期以及纤维脱落，纤维末端扭曲、变细。毛囊在第7天开始再生。相比之下，皮内注射生理盐水对毛囊活性没有影响。多次注射mEGF后，表皮比注射生理盐水后更厚且角化不全更明显，但在第14天时几乎恢复正常。多次注射mEGF后，皮脂腺大小持续有小幅增加（除皮内注射生理盐水所诱导的增加外），汗腺大小也有延迟的小幅增加。每周5天、持续4周每日在局部涂抹50%（v/v）丙二醇水溶液中的mEGF，对羊毛生长、毛囊及其他皮肤成分没有影响。观察到的唯一影响是，由于涂抹丙二醇水溶液，表皮角质层中角化细胞层数增加，细胞排列成清晰可辨的堆叠状。皮内注射mEGF所产生的影响表明，mEGF直接作用于皮肤的毛囊皮脂腺和表皮成分。
