Regeneration of wool follicles in autografts of sheep skin.

Three experiments involving six sheep were carried out at three different times in the annual wool growth cycle. Forty-eight full-thickness fitted autografts were sampled 2 h, 6 h, 24 h, 48 h, 3, 4, 5, 7, 9, 11, 14, 21 and 28 days after grafting. The histopathology of the grafts is described. Individual variation in 'take' was probably related to the speed with which vascular integrity was reestablished between graft and bed. Delayed acceptance caused the death of pilosebaceous units. In a successful graft, wool follicle regeneration appears to occur in three main ways: (1) an inactive follicle regenerates from the dormant bulb at about seven days, (2) follicles that were active at the time of grafting have first to form a papilla stalk, and such follicles did not become active until 11 days, (3) sometimes one or more outgrowths appeared at higher levels suggesting follicle regeneration by branching. In addition there were a few instances of de novo downgrowth of follicle plugs from the epidermis. Wool growth on the graft therefore arises almost entirely from the reorganisation of preexisting follicles.