Department of Respiratory Medicine, Zhuji Affiliated Hospital of Shaoxing University, Zhuji, Zhejiang 311800, P.R. China.
Department of Radiology, Zhuji Affiliated Hospital of Shaoxing University, Zhuji, Zhejiang 311800, P.R. China.
Mol Med Rep. 2020 Sep;22(3):1767-1774. doi: 10.3892/mmr.2020.11285. Epub 2020 Jun 26.
Transcription factor II B (TFIIB)‑related factor 2 (BRF2) is involved in the development of cancer, but its role in lung cancer is underreported. The present study aimed to explore the role of BRF2 in the regulation of lung cancer cells. Immunofluorescence staining and immunohistochemistry were performed to detect BRF2 protein expression in human lung cancer cells and tissues. Following cell transfection with small interfering RNA for silencing BRF2, the cell proliferation was examined by Cell Counting Kit‑8 and MTT assays. Cell apoptosis, migration and invasion were determined by flow cytometry, wound‑healing and Transwell assay. The expression levels of Akt, phosphorylated (p)‑Akt, Bax, E‑cadherin, Bcl‑2, N‑cadherin, Snail and epidermal growth factor receptor (EGFR) in human lung cancer A549 cells were detected by western blotting. The results demonstrated that BRF2 expression was increased in human lung cancer cells and tissues, and that silencing of BRF2 promoted cell apoptosis but inhibited cell proliferation and migration. The protein expression levels of Akt, E‑cadherin, p‑Akt, Bcl‑2, N‑cadherin, Snail and EGFR in A549 cells were inhibited by silencing of BRF2, while expression levels of Bax and E‑cadherin were increased by silencing BRF2. In conclusion, BRF2 demonstrates high expression in lung cancer and silencing of BRF2 inhibits the growth and metastasis of lung cancer cells. The current findings provide a novel approach for the treatment of lung cancer.
转录因子 II B（TFIIB）相关因子 2（BRF2）参与癌症的发生，但它在肺癌中的作用尚未得到充分报道。本研究旨在探讨 BRF2 在肺癌细胞调控中的作用。采用免疫荧光染色和免疫组织化学法检测人肺癌细胞和组织中 BRF2 蛋白的表达。用小干扰 RNA 沉默 BRF2 进行细胞转染后，通过细胞计数试剂盒-8 和 MTT 法检测细胞增殖。采用流式细胞术、划痕愈合和 Transwell 实验检测细胞凋亡、迁移和侵袭。采用 Western blot 检测人肺癌 A549 细胞中 Akt、磷酸化 Akt（p-Akt）、Bax、E-钙黏蛋白、Bcl-2、N-钙黏蛋白、Snail 和表皮生长因子受体（EGFR）的表达水平。结果表明，BRF2 在人肺癌细胞和组织中表达增加，沉默 BRF2 可促进细胞凋亡，抑制细胞增殖和迁移。沉默 BRF2 可抑制 A549 细胞中 Akt、E-钙黏蛋白、p-Akt、Bcl-2、N-钙黏蛋白、Snail 和 EGFR 的蛋白表达水平，同时增加 Bax 和 E-钙黏蛋白的表达水平。综上所述，BRF2 在肺癌中高表达，沉默 BRF2 可抑制肺癌细胞的生长和转移。本研究为肺癌的治疗提供了一种新的方法。
