Yadav Neelam, Singh Amit, Kaushik Mahima
Nano-bioconjugate Chemistry Lab, Cluster Innovation Centre, University of Delhi, Delhi, India.
Department of Chemistry, University of Delhi, Delhi, India.
J Mater Sci Mater Med. 2020 Jul 23;31(8):68. doi: 10.1007/s10856-020-06405-6.
Magnetic nanoparticles (MNPs) especially iron oxide (FeO) NPs have quite extensively been used for in vivo delivery of biomolecules and drugs because of their high bioconjugation efficiency. In this study, FeO NPs and (3-Aminopropyl) triethoxysilane (APTS) coated FeO NPs were synthesized and their interaction with Calf thymus (Ct) DNA has been studied in order to understand their usage in biomedical applications. Hydrothermal method was used for the NPs synthesis. Characterization of NPs was done using techniques like UV-Visible spectroscopy, FTIR spectroscopy, FE-SEM, EDAX, Zeta Sizer and powder XRD. Further, interaction studies of NPs with Ct-DNA were investigated using various physicochemical techniques. In UV-Visible studies, hypochromicity with binding constant 3.2 × 10 M was observed. Binding constants calculated using fluorescence studies were found to be k = 3.2 × 10 M, 2.9 × 10 M at 293 and 323 K respectively. Results of UV-Visible and fluorescence studies were in correlation with other techniques like UV-T and CD. All studies suggested alteration in DNA conformation on interaction with surface engineered FeO NPs, stabilizing DNA-NPs conjugate via partial intercalation and electrostatic interactions. This study may facilitate our understanding regarding the physicochemical properties and DNA-binding ability of APTS-FeO NPs for their further application in magnetosensitive biosensing and drug delivery. Iron oxide based magnetic nanoparticles are well known for their excellent bio-conjugation efficiency and therefore APTS-FeO NPs were synthesized via very simple and benign hydrothermal method. Further, the interaction of APTS-FeO NPs with calf thymus DNA was studied using various physicochemical techniques to explore their potential in biomedical applications.
磁性纳米颗粒（MNPs），尤其是氧化铁（FeO）纳米颗粒，因其高生物共轭效率而被广泛用于生物分子和药物的体内递送。在本研究中，合成了FeO纳米颗粒和（3-氨丙基）三乙氧基硅烷（APTS）包覆的FeO纳米颗粒，并研究了它们与小牛胸腺（Ct）DNA的相互作用，以了解它们在生物医学应用中的用途。采用水热法合成纳米颗粒。使用紫外-可见光谱、傅里叶变换红外光谱、场发射扫描电子显微镜、能谱分析、zeta粒度分析仪和粉末X射线衍射等技术对纳米颗粒进行表征。此外，使用各种物理化学技术研究了纳米颗粒与Ct-DNA的相互作用。在紫外-可见研究中，观察到结合常数为3.2×10⁶M时的减色现象。通过荧光研究计算出的结合常数在293K和323K时分别为k = 3.2×10⁶M、2.9×10⁶M。紫外-可见和荧光研究的结果与紫外-T和圆二色性等其他技术相关。所有研究表明，与表面工程化的FeO纳米颗粒相互作用时DNA构象发生改变，通过部分嵌入和静电相互作用稳定DNA-纳米颗粒共轭物。本研究可能有助于我们了解APTS-FeO纳米颗粒的物理化学性质和DNA结合能力，以便它们在磁敏生物传感和药物递送中进一步应用。基于氧化铁的磁性纳米颗粒以其优异的生物共轭效率而闻名，因此通过非常简单且温和的水热法合成了APTS-FeO纳米颗粒。此外，使用各种物理化学技术研究了APTS-FeO纳米颗粒与小牛胸腺DNA的相互作用以探索它们在生物医学应用中的潜力。
