Shahabadi Nahid, Falsafi Monireh, Mansouri Kamran
Department of Inorganic Chemistry, Faculty of Chemistry, Razi University, Kermanshah, Iran; Medical Biology Research Center (MBRC), Kermanshah University of Medical Sciences, Kermanshah, Iran.
Department of Inorganic Chemistry, Faculty of Chemistry, Razi University, Kermanshah, Iran.
Colloids Surf B Biointerfaces. 2016 May 1;141:213-222. doi: 10.1016/j.colsurfb.2016.01.054. Epub 2016 Feb 1.
In this study, Fe3O4@SiO2-cytarabine magnetic nanoparticles (MNPs) were prepared via chemical coprecipitation reaction and coating silica on the surface of Fe3O4 MNPs by Stöber method via sol-gel process. The surface of Fe3O4@SiO2 MNPs was modified by an anticancer drug, cytarabine. The structural properties of the samples were characterized by X-ray diffraction (XRD), Fourier transform infrared spectroscopy (FT-IR), Zetasizer analyzer, and transmission electron microscopy (TEM). The results indicated that the crystalline phase of iron oxide NPs was magnetite (Fe3O4) and the average sizes of Fe3O4@SiO2-cytarabine MNPs were about 23 nm. Also, the surface characterization of Fe3O4@SiO2-cytarabine MNPs by FT-IR showed that successful coating of Fe3O4 NPs with SiO2 and binding of cytarabine drug onto the surface of Fe3O4@SiO2 MNPs were through the hydroxyl groups of the drug. The in vitro cytotoxic activity of Fe3O4@SiO2-cytarabine MNPs was investigated against cancer cell line (HL60) in comparison with cytarabine using MTT colorimetric assay. The obtained results showed that the effect of Fe3O4@SiO2-cytarabine magnetic nanoparticles on the cell lines were about two orders of magnitude higher than that of cytarabine. Furthermore, in vitro DNA binding studies were investigated by UV-vis, circular dichroism, and fluorescence spectroscopy. The results for DNA binding illustrated that DNA aggregated on Fe3O4@SiO2-cytarabine MNPs via groove binding.
在本研究中，通过化学共沉淀反应制备了Fe3O4@SiO2-阿糖胞苷磁性纳米颗粒（MNPs），并采用溶胶-凝胶法通过Stöber法在Fe3O4 MNPs表面包覆二氧化硅。用抗癌药物阿糖胞苷对Fe3O4@SiO2 MNPs的表面进行修饰。通过X射线衍射（XRD）、傅里叶变换红外光谱（FT-IR）、Zetasizer分析仪和透射电子显微镜（TEM）对样品的结构性质进行了表征。结果表明，氧化铁纳米颗粒的晶相为磁铁矿（Fe3O4），Fe3O4@SiO2-阿糖胞苷MNPs的平均尺寸约为23 nm。此外，通过FT-IR对Fe3O4@SiO2-阿糖胞苷MNPs进行表面表征表明，Fe3O4纳米颗粒成功地被SiO2包覆，且阿糖胞苷药物通过药物的羟基与Fe3O4@SiO2 MNPs表面结合。与阿糖胞苷相比，采用MTT比色法研究了Fe3O4@SiO2-阿糖胞苷MNPs对癌细胞系（HL60）的体外细胞毒性活性。所得结果表明，Fe3O4@SiO2-阿糖胞苷磁性纳米颗粒对细胞系的作用比阿糖胞苷高约两个数量级。此外，通过紫外可见光谱、圆二色光谱和荧光光谱研究了体外DNA结合。DNA结合结果表明，DNA通过沟槽结合聚集在Fe3O4@SiO2-阿糖胞苷MNPs上。
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