Institute of Cell Biophysics of the Russian Academy of Sciences-Federal Research Center, Pushchino Scientific Center for Biological Research of the Russian Academy of Sciences, Pushchino, Russia.
Institute of Theoretical and Experimental Biophysics, Russian Academy of Sciences, Pushchino, Russia.
Appl Spectrosc. 2021 Feb;75(2):189-198. doi: 10.1177/0003702820949285. Epub 2020 Oct 8.
Analysis of structural and dynamic properties of water in suspensions of liposomes composed from 1,2-dipalmitoyl-sn-glycero-3-phosphocholine (DPPC) in three phase states (gel, rippled gel, liquid crystalline phase) by means of terahertz time-domain spectroscopy in 0.3-3.3 THz range was conducted in the current work. Fraction of free water molecules in DPPC liposome suspension was shown to decrease with temperature (compared to the analogous aqueous solution without liposomes), and intermolecular water binding was enhanced. The most crucial changes occur during gel-rippled gel phase transition (pretransition): at temperatures below pretransition point, liposomes alleviate water binding degree, while at temperatures above the transition point, they enhance water binding. This study has demonstrated the high information content of the terahertz time-domain spectroscopy method for exploring the hydration properties of phospholipids in water.
本工作通过 0.3-3.3 THz 太赫兹时域光谱分析了由 1,2-二月桂酰基-sn-甘油-3-磷酸胆碱（DPPC）组成的脂质体在三相状态（凝胶、波纹凝胶、液晶相）中的结构和动态特性。结果表明，与不含脂质体的类似水溶液相比，DPPC 脂质体悬浮液中的游离水分子分数随温度降低（与不含脂质体的类似水溶液相比），分子间水结合增强。最关键的变化发生在凝胶-波纹凝胶相变（预相变）期间：在低于预相变点的温度下，脂质体减轻水结合程度，而在高于相变点的温度下，脂质体增强水结合。这项研究表明，太赫兹时域光谱法对于研究磷脂在水中的水合性质具有很高的信息量。
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