Wang Xinmao, Zhang Yangruizi, Xie Maobin, Wang Zhibiao, Qiao Hai
State Key Laboratory of Ultrasound in Medicine and Engineering, College of Biomedical Engineering, Chongqing Medical University, Chongqing, 400016, China.
Chongqing Key Laboratory of Biomedical Engineering, Chongqing Medical University, Chongqing 400016, China.
Curr Issues Mol Biol. 2023 Apr 26;45(5):3757-3771. doi: 10.3390/cimb45050242.
The evolution of unicellular to multicellular life is considered to be an important step in the origin of life, and it is crucial to study the influence of environmental factors on this process through cell models in the laboratory. In this paper, we used giant unilamellar vesicles (GUVs) as a cell model to investigate the relationship between environmental temperature changes and the evolution of unicellular to multicellular life. The zeta potential of GUVs and the conformation of the headgroup of phospholipid molecules at different temperatures were examined using phase analysis light scattering (PALS) and attenuated total reflection-Fourier transform infrared spectroscopy (ATR-FTIR), respectively. In addition, the effect of increasing temperature on the aggregation of GUVs was further investigated in ionic solutions, and the possible mechanisms involved were explored. The results showed that increasing temperature reduced the repulsive forces between cells models and promoted their aggregation. This study could effectively contribute to our understanding of the evolution of primitive unicellular to multicellular life.
从单细胞生命到多细胞生命的演化被认为是生命起源中的重要一步，通过实验室中的细胞模型研究环境因素对这一过程的影响至关重要。在本文中，我们使用巨型单层囊泡（GUVs）作为细胞模型来研究环境温度变化与从单细胞生命到多细胞生命演化之间的关系。分别使用相分析光散射（PALS）和衰减全反射傅里叶变换红外光谱（ATR-FTIR）检测了不同温度下GUVs的ζ电位和磷脂分子头部基团的构象。此外，在离子溶液中进一步研究了温度升高对GUVs聚集的影响，并探讨了其中可能涉及的机制。结果表明，温度升高降低了细胞模型之间的排斥力并促进了它们的聚集。这项研究可以有效地帮助我们理解原始单细胞到多细胞生命的演化。
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